Smart Microneedles with Porous Polymer Layer for Glucose-Responsive Insulin Delivery
| Autor: | Sanghyun An, Hye Jin Choi, Gyu Man Kim, Asad Ullah, Mijin Jang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_treatment
sodium bicarbonate Pharmaceutical Science lcsh:RS1-441 02 engineering and technology engineering.material 010402 general chemistry 01 natural sciences Article lcsh:Pharmacy and materia medica chemistry.chemical_compound glucose-responsive Coating insulin delivery medicine Glucose oxidase Thin film chemistry.chemical_classification Sodium bicarbonate biology Insulin porous coated-microneedles Polymer 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Chemical engineering Gluconic acid engineering biology.protein 0210 nano-technology Layer (electronics) |
| Zdroj: | Pharmaceutics, Vol 12, Iss 606, p 606 (2020) Pharmaceutics Volume 12 Issue 7 |
| ISSN: | 1999-4923 |
| Popis: | A closed-loop system imitating the function of pancreatic cells, connected to microneedles (MNs) that automatically &ldquo release&rdquo insulin in response to the blood glucose (BG) levels would be highly satisfactory for improving the quality of life and health for diabetes patients. This paper describes an easy, fast and simple technique of coating a porous polymer layer on stainless steel (SS) MNs that release insulin in a glucose-responsive fashion. It was fabricated by sealing insulin, sodium bicarbonate (a pH-sensitive element [NaHCOз]) and glucose oxidase (glucose-specific enzymes [GOx]) into the pores of a porous polymer coating. Glucose can passively diffuse into the pores and become oxidized to gluconic acid by GOx, thereby causing a decrease in local pH. The subsequent reaction of protons with NaHCOз forms carbon dioxide (CO2) which creates pressure inside the pores, thereby rupturing the thin polymer film and releasing the encapsulated insulin. Field emission scanning electron microscopy (FE-SEM) images displayed that upon the exposure of MNs to glucose-free phosphate buffer saline (PBS) with pH 7.4, the pores of the porous MNs were closed, while in MNs exposed to a hyperglycemic glucose level, the pores were opened and the thin film burst. These MNs demonstrated both in vitro (in porcine skin and PBS) and in vivo (in diabetic rats) glucose-mediated insulin release under hyperglycemic conditions with rapid responsiveness. This study validated that the release of insulin from porous MNs was effectively correlated with glucose concentration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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