The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells
| Autor: | Ivana Novak, Hiroko Matsuda, Akihiro Inagaki, Mikio Hayashi |
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| Jazyk: | angličtina |
| Předmět: |
Male
0301 basic medicine Adenosine Physiology Clinical Biochemistry Aminopyridines Cystic Fibrosis Transmembrane Conductance Regulator Membrane Potentials chemistry.chemical_compound 0302 clinical medicine CFTR Receptor Adenosine receptor Purinergic signalling Cell biology Female Ion Channels Receptors and Transporters medicine.drug Anions medicine.medical_specialty Guinea Pigs Biology Receptor Adenosine A2B Cell Line 03 medical and health sciences Adenosine A1 receptor Chlorides Internal medicine Physiology (medical) Phenethylamines medicine Animals Humans Duct Rats Wistar Uridine Pancreas Transepithelial potential difference CGS-21680 Pancreatic Ducts Cl− channel Epithelial Cells Rats Cl channel 030104 developmental biology Endocrinology chemistry 030217 neurology & neurosurgery Adenosine A2B receptor |
| Zdroj: | Hayashi, M, Inagaki, A, Novak, I & Matsuda, H 2016, ' The adenosine A 2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells ', Pfluegers Archiv, vol. 468, no. 7, pp. 1171-1181 . https://doi.org/10.1007/s00424-016-1806-9 Europe PubMed Central Pflugers Archiv |
| ISSN: | 0031-6768 |
| DOI: | 10.1007/s00424-016-1806-9 |
| Popis: | Adenosine modulates a wide variety of biological processes via adenosine receptors. In the exocrine pancreas, adenosine regulates transepithelial anion secretion in duct cells and is considered to play a role in acini-to-duct signaling. To identify the functional adenosine receptors and Cl− channels important for anion secretion, we herein performed experiments on Capan-1, a human pancreatic duct cell line, using open-circuit Ussing chamber and gramicidin-perforated patch-clamp techniques. The luminal addition of adenosine increased the negative transepithelial potential difference (Vte) in Capan-1 monolayers with a half-maximal effective concentration value of approximately 10 μM, which corresponded to the value obtained on whole-cell Cl− currents in Capan-1 single cells. The effects of adenosine on Vte, an equivalent short-circuit current (Isc), and whole-cell Cl− currents were inhibited by CFTRinh-172, a cystic fibrosis transmembrane conductance regulator (CFTR) Cl− channel inhibitor. The adenosine A2B receptor agonist, BAY 60-6583, increased Isc and whole-cell Cl− currents through CFTR Cl− channels, whereas the A2A receptor agonist, CGS 21680, had negligible effects. The A2B receptor antagonist, PSB 603, inhibited the response of Isc to adenosine. Immunohistochemical analysis showed that the A2A and A2B receptors colocalized with Ezrin in the luminal membranes of Capan-1 monolayers and in rat pancreatic ducts. Adenosine elicited the whole-cell Cl− currents in guinea pig duct cells. These results demonstrate that luminal adenosine regulates anion secretion by activating CFTR Cl− channels via adenosine A2B receptors on the luminal membranes of Capan-1 cells. The present study endorses that purinergic signaling is important in the regulation of pancreatic secretion. |
| Databáze: | OpenAIRE |
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