Conditional Gene Expression in Human Intracranial Xenograft Tumors
| Autor: | Asadullah Khan, Shuguang Wang, Frederick F. Lang, Timothy S. Schaefer |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Tetracycline
Transplantation Heterologous Mice Nude Repressor General Biochemistry Genetics and Molecular Biology Mice Genes Reporter Glioma Gene expression Tumor Cells Cultured medicine Animals Humans Beta-galactosidase Gene Doxycycline biology Brain Neoplasms beta-Galactosidase medicine.disease Anti-Bacterial Agents Gene Expression Regulation Neoplastic Cell culture Immunology Cancer research biology.protein Neoplasm Transplantation Biotechnology medicine.drug |
| Zdroj: | BioTechniques. 31:196-202 |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/01311dd04 |
| Popis: | In an initial effort to determine the effect of expressing potentially therapeutic gene products on the growth properties of glioma tumor xenografts, we describe the development of cell lines that can conditionally express β-galactosidase (β-gal). To achieve this, we generated stable cell lines that express the modified tetracycline repressor molecule (rtTA) and the β-gal gene under control of tetracycline-responsive cis-elements. The resulting cell lines express functional β-gal following treatment with the tetracycline analog doxycyclin (Dox). These cells were then used to form intracranial tumors after injection into the brain using an implantable guide-screw system. The xenografts were found to express β-gal when the animals were fed drinking water containing Dox. From these studies, we conclude that the expression of a target gene in a human xenograft growing in the brain of a living mouse can be conditionally regulated. |
| Databáze: | OpenAIRE |
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