Plasma mitochondrial DNA levels are inversely associated with HIV-RNA levels and directly with CD4 counts: potential role as a biomarker of HIV replication
| Autor: | Álvaro Mena, Manuel Crespo, Ignacio Rego-Pérez, Francisco J. Blanco, S. Relaño, Vanesa Balboa, Andrés Tabernilla, Marta Grandal, Berta Pernas, Eva Poveda, Ángeles Castro-Iglesias |
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| Přispěvatelé: | Pharmaceutical and Pharmacological Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Virus replication Cell Human immunodeficiency virus (HIV) HIV Infections medicine.disease_cause Virus Replication DNA Mitochondrial/blood Cohort Studies Plasma 0302 clinical medicine Pharmacology (medical) 030212 general & internal medicine Genetics CD4 count determination procedure Plasma samples virus diseases Middle Aged Viral Load Mitochondrial Infectious Diseases medicine.anatomical_structure HIV Infections/epidemiology Spain/epidemiology Biomarker (medicine) RNA Viral Female Microbiology (medical) Adult Mitochondrial DNA HIV-1/genetics Viremia Real-Time Polymerase Chain Reaction DNA Mitochondrial Andrology 03 medical and health sciences Blood HIV RNA Virology medicine Humans Biological makers RNA Viral/blood Pharmacology business.industry HIV DNA medicine.disease HIV infection CD4 Lymphocyte Count 030104 developmental biology Viral replication Spain HIV-1 business Biomarkers/blood Biomarkers Linear trend |
| Zdroj: | RUC. Repositorio da Universidade da Coruña instname |
| Popis: | [Abstract] Objectives. To evaluate plasma mitochondrial DNA (mtDNA) levels among HIV-infected patients and its potential role as a biomarker of residual viral replication. Methods. HIV-infected patients on follow-up at a reference hospital in north-west Spain were selected. DNA was isolated from plasma samples and mtDNA levels were assessed using a quantitative real-time PCR assay. HIV-RNA levels and CD4+ cell counts were evaluated in the same blood samples used for plasma mtDNA quantification. Epidemiological and clinical variables were included for the analysis. Results. A total of 235 HIV-infected patients were included. Mean plasma mtDNA levels were 217 ± 656 copies/μL for naive (31.9%) and 364 ± 939 copies/μL for HIV-infected patients receiving ART and with suppressed viraemia (P = 0.043). Among the latter, mean plasma mtDNA levels were 149 ± 440 copies/μL for those with low-level viraemia (LLV; HIV-RNA 20–200 copies/mL), 265 ± 723 copies/μL for those with detected-not-quantified (DNQ) viraemia (HIV-RNA |
| Databáze: | OpenAIRE |
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