Late-phase ischemic preconditioning in skeletal muscle: is the phenomenon protective?
| Autor: | Claudio Maldonado, Edwin E. Quan, John H. Barker, Santiago Ramirez, Taskin Tecimer, Federico V. Grossi |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Time Factors Necrosis Ischemia Muscle flap Surgical Flaps Rats Sprague-Dawley Late phase parasitic diseases medicine Animals cardiovascular diseases Ischemic Preconditioning Muscle Skeletal Total ischemia business.industry Latissimus dorsi muscle Skeletal muscle medicine.disease Rats medicine.anatomical_structure Reperfusion Injury Anesthesia Ischemic preconditioning Surgery medicine.symptom business |
| Zdroj: | Microsurgery. 24:151-156 |
| ISSN: | 1098-2752 0738-1085 |
| Popis: | Reports in the literature on the effectiveness of late-phase Ischemic preconditioning (IPC) in skeletal muscle are controversial. The purpose of this study was to determine in the same muscle flap model the effectiveness of various IPC protocols in inducing late-phase protection. Rat latissimus dorsi muscle (LDM) flaps were preconditioned with either 30 or 60 min of total ischemia, divided as follows: single cycles of either 30 or 60 min, two cycles of 15 or 30 min, and three cycles of 10 or 20 min. Ischemia cycles were separated by 10 min of reperfusion. A day after IPC, flaps were elevated and challenged with 4 h of ischemia. Three days later, flaps were assessed for viability. We found that IPC protocols of different total durations and comprised of two or three cycles of ischemia elicited a protective effect against necrosis. We conclude that IPC induces late-phase protection against necrosis in skeletal muscle, and that the protection requires more than one ischemia/reperfusion cycle. |
| Databáze: | OpenAIRE |
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