Locus ABO (9q 34.1) genotyping in blood donors in the state of Sao Paulo, Brazil

Autor: Juliana R. Cintra, Luiz Carlos de Mattos, Haroldo Wilson Moreira, Fábio E. Sanchez, Andréa B. C. F. Salles, Claudia Regina Bonini-Domingos
Přispěvatelé: Faculdade de Medicina de São José do Rio Preto (FAMERP), Universidade Estadual Paulista (Unesp)
Jazyk: portugalština
Rok vydání: 2001
Předmět:
Zdroj: Revista Brasileira de Hematologia e Hemoterapia v.23 n.1 2001
Revista brasileira de hematologia e hemoterapia
Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)
instacron:ABHHTC
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Revista Brasileira de Hematologia e Hemoterapia, Volume: 23, Issue: 1, Pages: 15-22, Published: APR 2001
Revista Brasileira de Hematologia e Hemoterapia, Vol 23, Iss 1, Pp 15-22 (2001)
Popis: The advances of molecular genetics enabled usto understand the molecular basis of the ABOlocus. Considering its importance as a geneticmarker and its applications, the aim of this studywas to verify the distribution of the ABO genotypesin a Brazilian population from the Northwestregion of the Sao Paulo State, Brazil. Thegenomic DNA was extracted from three hundredand twenty four healthy Brazilian blood donors(O 150; A 118; B 32 and AB 24) and analyzedby PCR amplification followed by restrictionenzyme digestion. Fourteen genotypes wereidentified and the relative frequencies of the O 1 ,O 1v , O 2 , A and B genes were estimated at 44.6%,16.9%, 4.1%, 25.3% and 9.1%, respectively.These results demonstrate that the ABO locuspresents a high polymorphism as revealed bymolecular analysis.Rev.bras.hematol.hemoter., 2001, 23(1): 15-22Key words: ABO genotypes, ABO genes, blooddonorsReferencias Bibliograficas1. Watkins WM. Biochemistry and genetics ofthe ABO, Lewis and P blood group systems.Adv Hum Genet, 1980; 10: 1-136.2. Daniels G. Human Blood Groups,Cambridge, 1995, 737p.3. Yamamoto F, Marken J, Tsuji T, White T,Clausen H, Hakomori S. Cloning andcharacterization of DNA complementary tohuman UDP-GalNAc: Fuc-alfa 1-2-alfa 1-3 GalNAc transferase (Histo-blood groupA transferase) mRNA. J Biol Chem, 1990;265 (2): 1146-1151.4. Yamamoto F, Clausen H, White T, MarkenJ, Hakomori S. Molecular genetic basis of thehisto-blood group ABO system. Nature, 1990;345: 229-233.5. Yamamoto F, Hakomori S. Sugar-nucleotidedonor specificity of histo-blood group A andB transferases is based on amino acidsubstitutions. J Biol Chem; 1990; 265(31):19257-19262.6. Chang JG, Lee LS, Chen PH. Rapidgenotyping of the ABO blood group. Blood,1992; 79(8): 2176-2177.7. Lee JCI, Chang JG. ABO genotyping bypolymerase chain reaction. J Foren Sci,1992; 37(5): 1269-1275.8. Crouse C, Vincek V. Identification of ABO alleleson forensic type specimens using rapid ABOgenotyping. BioTechnics, 1995; 18(3): 478-482.9. Liechti-Gallati S, Neeser D. Efficient andreliable PCR based detection of the ABO bloodgroup alleles: genotyping on stamps and otherbiological evidence samples. J Forens Sci,1996; 41(4): 653-657.10.Gassner C, Schmarda A, Nussbaumer W,Schonitzer D. ABO glycosyltransferasegenotyping by polymerase chain reactionusing sequence-specific primer. Blood, 1996;88(5): 1852-1856.11.Yamamoto F, McNeill PD, Yamamoto M,Hakomori S, Bromilow IM, Duguid JKM.Molecular genetic analysis of the ABO bloodgroup system: 4. Another type of O allele. VoxSang, 1993; 64: 175-178.12.Grunnett N, Steffelson R, Bennett EP, ClausenH. Evaluation of histo-blood group ABOgenotyping in a danish population:frequency of a novel O allele defined as O
Databáze: OpenAIRE
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