Macrophage-mediated killing of splenic lymphocytes in adjuvant-induced arthritis
| Autor: | A. C. Hanglow, John W. Coffey, H. Bachmann |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Allergy medicine.medical_specialty Immunology Arthritis Spleen In Vitro Techniques Toxicology chemistry.chemical_compound Lactate dehydrogenase Internal medicine Concanavalin A Splenocyte medicine Animals Macrophage Pharmacology (medical) Lymphocytes Pharmacology Cell Death L-Lactate Dehydrogenase business.industry Macrophages Rats Inbred Strains medicine.disease Arthritis Experimental Molecular biology Adjuvant induced arthritis Rats Endocrinology medicine.anatomical_structure chemistry Mitogens Thymidine business Cell Division |
| Zdroj: | Agents and Actions. 34:11-15 |
| ISSN: | 1420-908X 0065-4299 |
| DOI: | 10.1007/bf01993224 |
| Popis: | After 72 hrs in culture, unseparated spleen cells from rats with adjuvant-induced arthritis (AA) stimulated with high concentrations of Con A (greater than 0.63 micrograms/ml) leaked more lactate dehydrogenase (LDH) than did either unstimulated cultures or cultures stimulated with lower concentrations of Con A. This reflects an increase in dead or dying cells at concentrations of Con A greater than 0.63 micrograms/ml. Since Con A did not increase LDH leakage in macrophage-depleted cultures of AA splenocytes, the decreased viability in unseparated, Con A-stimulated cultures appears to be a macrophage-dependent phenomenon. Since the increase in LDH release at high concentrations of Con A paralleled the decrease in [3H]-thymidine incorporation, these results suggest that Con A (greater than 0.63 micrograms/ml) induces macrophages from AA rats to kill splenic lymphocytes in culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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