Evolution of the theoretical description of the isoelectric focusing experiment: III. Carrier ampholyte behavior in transient, bidirectional isotachophoresis

Autor: Gyula Vigh, Bohuslav Gaš
Rok vydání: 2023
Předmět:
Zdroj: ELECTROPHORESIS. 44:689-700
ISSN: 1522-2683
0173-0835
DOI: 10.1002/elps.202200239
Popis: In modern isoelectric focusing (IEF) systems, where (i) convective mixing is prevented by gels or small cross-sectional area separation channels, (ii) current densities vary spatially due to the presence of electrode vessels with much larger cross-sectional areas than those of the gels or separation channels, and (iii) electrophoretic and diffusive fluxes do not balance each other, stationary, steady state pH gradients cannot form (open system IEF). Open system IEF is currently described as a two-stage process: a rapid IEF process forms the pH gradient from the carrier ampholytes (CAs) in the first stage, then isotachophoretic processes degrade the pH gradient in the second stage as the extreme pI CAs are moved into the electrode vessels where they become diluted. Based on the ratios of the local effective mobilities and the local conductivities (
Databáze: OpenAIRE
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