Use of Vaxfectin Adjuvant with DNA Vaccine Encoding the Measles Virus Hemagglutinin and Fusion Proteins Protects Juvenile and Infant Rhesus Macaques against Measles Virus
| Autor: | Fernando P. Polack, Chien Hsiung Pan, Nitya Nair, Qun Wei, Adrian Vilalta, Gretchen S. Jimenez, Alain Rolland, Robert J. Adams, Diane E. Griffin |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
T-Lymphocytes Measles Vaccine Molecular Sequence Data Clinical Biochemistry Immunology Hemagglutinins Viral Hemagglutinin (influenza) Viremia Antibodies Viral Measles DNA vaccination Measles virus Interferon-gamma Mice Adjuvants Immunologic Neutralization Tests Vaccines DNA medicine Animals Humans Immunology and Allergy Neutralizing antibody Mice Inbred BALB C biology Phosphatidylethanolamines Vaccination Sequence Analysis DNA Vaccine Research biology.organism_classification medicine.disease Macaca mulatta Virology biology.protein Female Measles vaccine Viral Fusion Proteins |
| Zdroj: | Clinical and Vaccine Immunology. 15:1214-1221 |
| ISSN: | 1556-679X 1556-6811 |
| Popis: | A measles virus vaccine for infants under 6 months of age would help control measles. DNA vaccines hold promise, but none has provided full protection from challenge. Codon-optimized plasmid DNAs encoding the measles virus hemagglutinin and fusion glycoproteins were formulated with the cationic lipid-based adjuvant Vaxfectin. In mice, antibody and gamma interferon (IFN-γ) production were increased by two- to threefold. In macaques, juveniles vaccinated at 0 and 28 days with 500 μg of DNA intradermally or with 1 mg intramuscularly developed sustained neutralizing antibody and H- and F-specific IFN-γ responses. Infant monkeys developed sustained neutralizing antibody and T cells secreting IFN-γ and interleukin-4. Twelve to 15 months after vaccination, vaccinated monkeys were protected from an intratracheal challenge: viremia was undetectable by cocultivation and rashes did not appear, while two naïve monkeys developed viremia and rashes. The use of Vaxfectin-formulated DNA is a promising approach to the development of a measles vaccine for young infants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|