Isolation, purification and characterization of RNA polymerases from wheat germ
| Autor: | Jerome J. Jendrisak, Wayne M. Becker |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Time Factors
Tritium Biochemistry Genetics and Molecular Biology (miscellaneous) Peptides Cyclic Vibration Chromatography DEAE-Cellulose chemistry.chemical_compound Drug Stability Magnesium Polymerase Triticum chemistry.chemical_classification Oligopeptide Manganese biology Basidiomycota Osmolar Concentration RNA DNA DNA-Directed RNA Polymerases Templates Genetic Mycotoxins Plants Ribonucleoside Isoenzymes Enzyme chemistry Biochemistry Ionic strength Ammonium Sulfate biology.protein Chromatography Gel Oligopeptides |
| Zdroj: | Biochimica et biophysica acta. 319(1) |
| ISSN: | 0006-3002 |
| Popis: | Two DNA-dependent RNA polymerases (ribonucleoside triphosphate: RNA nucleotidyltransferase, EC 2.7.7.6) have been isolated from wheat germ. The enzymes were solubilized by high salt extraction with sonication and were resolved by DEAE-cellulose chromatography. Enzyme I eluted at 0.11 M (NH 4 ) 2 SO 4 , was insensitive to α-amanitin, and was relatively labile. Enzyme II eluted at 0.22 M (NH 4 ) 2 SO 4 , was inhibited by α-amanitin, and was stable after DEAE-cellulose chromatography. Both enzymes were more active with denatured than native DNA as template, but differed in their relative activities with Mg 2+ and Mn 2+ and in their ionic strength requirements. The quiescent wheat embryo appears to be a rich source of RNA polymerases and their properties are similar to nuclear RNA polymerases from other eukaryotes. |
| Databáze: | OpenAIRE |
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