Increased nitric oxide (NO) pathway metabolites in the vitreous fluid of patients with rhegmatogenous retinal detachment or diabetic traction retinal detachment

Autor: Hans M.H. van Eijk, Alfons G.H. Kessels, Ellen C. La Heij, Roselie M.H. Diederen, Nicolaas E. P. Deutz, Fred Hendrikse
Přispěvatelé: Ophthalmology, Oogheelkunde, Algemene Heelkunde, MUMC+: KIO Kemta (9), RS: NUTRIM School of Nutrition and Translational Research in Metabolism
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Male
medicine.medical_specialty
Arginine
genetic structures
Nitric Oxide Synthase Type II
Nitric Oxide
Nitric oxide
Pathogenesis
Retinal Detachment/metabolism
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Arginine/metabolism
Diabetic Retinopathy/metabolism
Downregulation and upregulation
Ophthalmology
medicine
Citrulline
Humans
Nitrites
Chromatography
High Pressure Liquid
Aged
Retina
Chromatography
Diabetic Retinopathy
Citrulline/metabolism
Vitreous Body/metabolism
business.industry
Nitrites/metabolism
Retinal Detachment
Retinal detachment
Diabetic retinopathy
Middle Aged
medicine.disease
Sensory Systems
eye diseases
Surgery
Vitreous Body
medicine.anatomical_structure
chemistry
High Pressure Liquid
Nitric Oxide/metabolism
Female
sense organs
business
Nitric Oxide Synthase Type II/metabolism
Signal Transduction
Zdroj: Graefe s archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 244(6), 683-8. Springer Verlag
Graefe's Archive for Clinical and Experimental Ophthalmology, 244(6), 683-688. Springer Verlag
ISSN: 0721-832X
DOI: 10.1007/s00417-005-0141-1
Popis: BACKGROUND: Nitric oxide (NO) plays a significant role in physiological and pathological processes in the retina. In the L-arginine-NO pathway, NO synthase (NOS) converts L-arginine to NO and L-citrulline. Increased NO production, mediated by inducible NOS has been implicated in the pathogenesis of various vitreoretinal diseases. In the present study it is hypothesized that in rhegmatogenous retinal detachment (RRD), the production of NO pathway metabolites might be upregulated. METHODS: Using high-pressure liquid chromatography citrulline, arginine and nitrite were measured in vitreous fluid of 93 eyes with RRD, nine eyes with a traction retinal detachment due to proliferative diabetic retinopathy (PDR), and in 49 control samples of vitreous fluid from eyes without retinal detachment. RESULTS: The mean vitreous concentrations of citrulline and arginine were significantly increased in eyes with RRD (9.6+/-4.3 and 97.3+/-29.2; respectively) or in eyes with a traction retinal detachment (25.8+/-10.3 and 130.7+/-23.7; respectively) as compared to control eyes (7.1+/-3.2 and 75.9+/-18.1; respectively). The mean level of nitrite was also higher in vitreous fluid of patients with RRD (2.24+/-1.4) or patients with a traction retinal detachment (2.21+/-0.72) than in the controls (2.01+/-0.72), although not significantly so. CONCLUSIONS: We found increased levels of NO pathway metabolites in the vitreous fluid of eyes with retinal detachment, which may reflect a possible role of NO in the pathogenesis of this disease.
Databáze: OpenAIRE
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