First detection and genetic characterization of equid herpesvirus 2, 4, and 5 in China
| Autor: | Ling Kuang, Panpan Tong, Xiaozhen Song, Chenyang Jia, Jinxin Xie, Lei Zhang, Li Zhang, Meiling Ren, Nuerlan Palidan |
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| Rok vydání: | 2020 |
| Předmět: |
Viral metagenomics
medicine.medical_specialty China Sequence analysis Respiratory Tract Diseases Biology Disease Outbreaks 03 medical and health sciences Open Reading Frames Medical microbiology Virology medicine Prevalence Animals Equine herpesvirus 2 Horses Herpesviridae Phylogeny 030304 developmental biology 0303 health sciences 030306 microbiology Coinfection Respiratory disease Outbreak Genetic Variation General Medicine Herpesviridae Infections medicine.disease biology.organism_classification Metagenomics DNA Viral Horse Diseases |
| Zdroj: | Archives of virology. 166(5) |
| ISSN: | 1432-8798 |
| Popis: | In November 2018, an outbreak of respiratory disease occurred in foals at an equestrian club in Changji, northern Xinjiang, China. We applied viral metagenomics to investigate this outbreak and identify potential pathogens involved in this equine respiratory syndrome. The metagenomics data revealed the presence of sequences matching those of equid herpesvirus (EHV) 2, 4, and 5. PCR with specific primers targeting ORF33 of EHV-4 and ORF8 of EHV-2 and EHV-5 revealed coinfection with these viruses in this respiratory syndrome. To investigate the prevalence of these viruses in China, 453 nasal swabs from clinically healthy thoroughbred foals (36/453, 7.9%) and horses (417/453, 92.1%) were collected from several equestrian clubs. Forty-five (9.9%) of the samples tested positive for EHV-5 DNA, and seven (1.5%) tested positive for EHV-2, but all were negative for EHV-4 DNA. Forty-nine (10.8%) samples tested positive for both EHV-5 and EHV-2 DNA. Using these samples, one complete EHV-4 ORF33, 10 partial EHV-2 ORF8, and 50 partial EHV-5 ORF8 sequences from the 10 diseased foals and 50 thoroughbred horses were then determined. Sequence analysis indicated that EHV-4 ORF33 and EHV-5 ORF8, in contrast to EHV-2 ORF8, had high sequence similarity to those of published sequences. Our data provide the first evidence that EHV-2, -4, and -5 co-circulate in China and that EHV-4 is potentially involved in this respiratory disease in foals. |
| Databáze: | OpenAIRE |
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