Rapid identification ofPseudomonas aeruginosaby pulsed-field gel electrophoresis

Autor:	Nashwa Hagagy, Iman M El Kholy, Mohamed H. Abdel Aziz, Sahar El Alfay, Samy Selim
Rok vydání:	2014
Předmět:	Gel electrophoresis Strain (chemistry) Pseudomonas aeruginosa Biology medicine.disease_cause Single strain Microbiology Rapid identification Restriction enzyme pulsed-field gel electrophoresis Genotype medicine Pulsed-field gel electrophoresis Egypt Article Medical Biotechnology Biotechnology
Zdroj:	Biotechnology, Biotechnological Equipment
ISSN:	1314-3530 1310-2818
Popis:	Twenty clinical Pseudomonas aeruginosa isolates recovered from patients admitted to The General Hospital in Ismailia Governorate (Egypt) were examined in this study. We analysed P. aeruginosa ATCC 9027 (as a control strain) and 19 of the isolates after digestion with SpeI restriction endonuclease. After this we conducted a pulsed-field gel electrophoresis (PFGE) and typed the obtained 10 unique patterns, designated as A, A1, B, B1, C, C1, D, D1, E and F. We evaluated the genetic relatedness between all strains, based on ≥87% band identity. As a result, the isolates were grouped in the 10 clusters as follows: patterns A, A1, B, B1, C contained two strains each and patterns C1, D, D1, E contained a single strain each; the five remaining strains were closely related (genomic pattern F). One isolate belonged to antibiotype ‘b’. The genotype patterns of the P. aeruginosa ATCC 9027 control strain and isolate no. 11 were closely related and had two different antibiotypes ‘d’ and ‘c’, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7330d31e59eafa0be3d10038ac0f5c8e https://doi.org/10.1080/13102818.2014.981065 Zobrazit plný text záznamu