| Popis: |
This study aimed to investigate the effects of high-fat diet (HFD) berberine supplementation on growth, lipid metabolism, antioxidant capacity and lipometabolism-related genes expression of AMPK signaling pathway in juvenile black carp (Mylopharyngodon piceus). five hundred and forty healthy fish (4.04 ± 0.01 g) were randomly distributed into six groups, and fed six experimental diets: normal-fat diet (NFD, 5% fat), HFD (15% fat), and four HFDs supplemented with graded levels of berberine for 60 days, respectively. The results showed that, compared with fish fed NFD, HFD had no effects on the growth performance of fish except for reducing the survival rate, whereas HFD caused extensive lipid accumulation, oxidative stress injury and hepatic abnormalities. However, compared with the HFD group, Fish fed HFD supplemented with berberine at 98.26 or 196.21 mg/kg significantly improved the growth performance, increased serum high density lipoprotein-cholesterol (HDL-C) content, the activities of hepatic lipid metabolizing enzymes of hepatic lipase (HL), lipoprotein lipase (LPL), total lipase (TL), malate dehydrogenase (MDH) and hormone-sensitive lipase (HSL), liver antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) activities, and liver total antioxidant capacity (T-AOC) and reduced-glutathione (GSH) levels, and up-regulated the mRNA expression levels of adenosine-activated protein kinase (AMPK) subunits and lipolysis genes such as peroxisome proliferator-activated receptor alpha (PPARα), carnitine palmitoyltransferases 1 (CPT-1), acyl-coenzyme A oxidase (ACOX) and HSL (P P |
