Resistance to Pepper Mild Mottle Tobamovirus Conferred by the 54-kDa Gene Sequence in Transgenic Plants Does Not Require Expression of the Wild-Type 54-kDa Protein
| Autor: | Francisco Tenllado, José Ramón Díaz-Ruíz, María Teresa Serra, Isabel García-Luque |
|---|---|
| Přispěvatelé: | Dirección General de Investigación Científica y Técnica, DGICT (España) |
| Rok vydání: | 1996 |
| Předmět: |
Genes
Viral biology Transgene Tobamovirus fungi Wild type Gene Expression food and beverages Nicotiana benthamiana Genetically modified crops Plant disease resistance Plants Genetically Modified RNA-Dependent RNA Polymerase biology.organism_classification medicine.disease Virology Plants Toxic Plant virus Tobacco medicine RNA Viral Mottle Plant Diseases |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1996.0257 |
| Popis: | We previously reported thatNicotiana benthamianaplants transformed with the wild-type 54-kDa region of the pepper mild mottle tobamovirus, S strain (PMMoV-S), displayed two different resistance responses against PMMoV infection. Some of the transgenic plants exhibited a complete and highly resistant phenotype while the remaining plants showed a delayed resistance (Tenlladoet al.,1995,Virology211, 170–183). Here we show that some of theN. benthamianaplants transformed with a construct expressing a PMMoV-S truncated 54-kDa protein coding sequence also displayed a complete and highly resistant phenotype similar to that shown by the wild-type 54-kDa transgenic plants. This result indicates that the wild-type, full-length 54-kDa protein is not required in mediating the complete resistance phenotype against PMMoV. The remaining truncated 54-kDa transgenic plants were susceptible to PMMoV infection but showed a variable delay in the appearance of symptoms. Unlike the wild-type 54-kDa transgenic plants, which were initially susceptible to the infection but recovered later, the truncated 54-kDa transgenic plants never exhibited this delayed resistance phenotype. However, they displayed a new type of altered symptomatic phenotype. The truncated 54-kDa transgenic lines also exhibited a lower level of transgenic transcripts compared to the wild-type 54-kDa transgenic lines which could account for the absence of the delayed resistance phenotype. This work was supported by Grants AGF94-0364 and PB94-0023 from the Dirección General de Investigación Científica y Técnica (DGICYT). |
| Databáze: | OpenAIRE |
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