Overexpression of LeNHX4 improved yield, fruit quality and salt tolerance in tomato plants (Solanum lycopersicum L.)
| Autor: | María Nieves Aranda, Mostapha Maach, María Elena Sánchez, Kees Venema, Mourad Baghour, María Pilar Rodríguez-Rosales, Mustapha Akodad, Francisco Javier Gálvez |
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| Přispěvatelé: | Junta de Andalucía, Ministerio de Economía, Industria y Competitividad (España), National Centre for Scientific and Technical Research (Morocco) |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Sodium-Hydrogen Exchangers Proline Antiporter medicine.medical_treatment LeNHX4 antiporter Soluble sugars Plant Roots Salt Stress Antiporters 03 medical and health sciences 0302 clinical medicine Solanum lycopersicum Gene Expression Regulation Plant Genetics medicine Gene silencing Molecular Biology Gene Saline Plant Proteins biology Arabidopsis Proteins Chemistry Protein Sodium fungi food and beverages Salt Tolerance General Medicine Plants Genetically Modified biology.organism_classification Salinity tolerance Plant Leaves Salinity Horticulture 030104 developmental biology Fruit 030220 oncology & carcinogenesis Yield (chemistry) Potassium Solanum |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1573-4978 |
| Popis: | The function of the tomato K, Na/H antiporter LeNHX4 has been analyzed using 35S-driven gene construct for overexpressing a histagged LeNHX4 protein in Solanum lycopersicum L. Compared to wild-type plants, the expression of LeNHX4 was enhanced in most of plants transformed with a gene construct for LeNHX4 overexpression although some plants showed a decreased LeNHX4 expression. Overexpression of LeNHX4 was associated to an increased fruit size while silencing of this gene was related to a decreased fruit size. We have investigated the effect of LeNHX4 overexpression on fruit production and quality and we have also evaluated salt tolerance in two different overexpression lines by measuring proline, protein and glucose concentrations in tomato leaves grown either under control (0 mM NaCl) or saline (125 mM NaCl) conditions. Plants overexpressing LeNHX4 showed a higher amount of fruits than WT plants and accumulated higher contents of sugars and cations (Na and K). The application of 125 mM NaCl, affected negatively fruit production and quality of WT plants. However the transgenic lines overexpressing LeNXH4 increased fruit quality and yield. In relation to salt tolerance, overexpression lines showed higher levels of leaf proline, glucose and proteins under NaCl treatment. The overexpression of LeNHX4 in tomato plants, improved salinity tolerance and increased fruit yield and quality under both normal and salinity stress conditions. This work was supported by Grants from Consejería de Economía, Innovación, Ciencia y Empresa, Junta de Andalucía, Spain (CVI-7558 to MPRR), Spanish Ministry of Economy and Competitiveness and Agencia Estatal de Investigación (BIO2015-65056-P, BIO2016-81957-REDT/AEI and Programa I-COOPB+2013 Ref. COOPB20053 National Centre for Scientific and Technical Research COOPB20053). and Minister for Higher Education, Scientific Research and Executive Training (Morocco). |
| Databáze: | OpenAIRE |
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