Variation in sequences containing microsatellite motifs in the perennial biomass and forage grass, Phalaris arundinacea (Poaceae)
Autor: | Susanne Barth, Marta Jolanta Jankowska, Manfred Klaas, Tia Vellani, Trevor R. Hodkinson |
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Přispěvatelé: | European Union, KBBE-2011-5-289461). |
Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine Microsatellite markers Genotype Forage Biology Poaceae 01 natural sciences Polymorphism Single Nucleotide General Biochemistry Genetics and Molecular Biology Crop 03 medical and health sciences Phalaris arundinacea Botany Genetic variation Correspondence Reed canary grass Biomass Phalaris Nucleotide Motifs Medicine(all) 2. Zero hunger Expressed Sequence Tags Biomass (ecology) Base Sequence Biochemistry Genetics and Molecular Biology(all) food and beverages Genetic Variation General Medicine 15. Life on land biology.organism_classification SSR 030104 developmental biology Agronomy Genetic structure Microsatellite 010606 plant biology & botany Microsatellite Repeats |
Zdroj: | BMC Research Notes |
ISSN: | 1756-0500 |
Popis: | This study has been financed under the collaborative European Community Framework FP7 project GrassMargins (KBBE-2011-5-289461). peer-reviewed Forty three microsatellite markers were developed for further genetic characterisation of a forage and biomass grass crop, for which genomic resources are currently scarce. The microsatellite markers were developed from a normalized EST-SSR library. All of the 43 markers gave a clear banding pattern on 3 % Metaphor agarose gels. Eight selected SSR markers were tested in detail for polymorphism across eleven DNA samples of large geographic distribution across Europe. The new set of 43 SSR markers will help future research to characterise the genetic structure and diversity of Phalaris arundinacea, with a potential to further understand its invasive character in North American wetlands, as well as aid in breeding work for desired biomass and forage traits. P. arundinacea is particularly valued in the northern latitude as a crop with high biomass potential, even more so on marginal lands. European Community Framework FP7 |
Databáze: | OpenAIRE |
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