Performance of 2 commercial real-time polymerase chain reaction assays for the detection of Aspergillus and Pneumocystis DNA in bronchoalveolar lavage fluid samples from critical care patients
| Autor: | Elena Righi, William Gennari, Annunziata La Regina, Claudia Venturelli, Marco Machetti, Monica Pecorari, Elisabetta Blasi, Carlotta Francesca Orsi |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Adult Male Critical Care Biology Aspergillosis Real-Time Polymerase Chain Reaction law.invention Microbiology chemistry.chemical_compound law Predictive Value of Tests medicine Pneumocystis jirovecii Humans DNA Fungal Polymerase chain reaction Aged Retrospective Studies Aspergillus medicine.diagnostic_test Pneumocystis Pneumonia Pneumocystis General Medicine Middle Aged biology.organism_classification medicine.disease respiratory tract diseases Molecular Typing Pneumonia Infectious Diseases Bronchoalveolar lavage Real-time polymerase chain reaction chemistry ROC Curve Female Bronchoalveolar Lavage Fluid DNA |
| Zdroj: | Diagnostic microbiology and infectious disease. 73(2) |
| ISSN: | 1879-0070 |
| Popis: | This article investigates the performance of 2 commercial real-time polymerase chain reaction (PCR) assays, MycAssay™ Aspergillus (Myc Asp Assay) and MycAssay™ Pneumocystis (Myc PCP Assay), on the ABI 7300 platform for the detection of Aspergillus (Asp) or Pneumocystis jirovecii (Pj) DNA in bronchoalveolar lavage (BAL) samples from 20 patients. Operationally, patients enrolled were clustered into 3 groups: invasive aspergillosis group (IA, 7 patients), Pj pneumonia group (PCP, 8 patients), and negative control group (5 patients). All the IA patients were Myc Asp Assay positive, whereas 12 non-IA patients returned negative PCR results. Furthermore, 7 of 8 PCP patients were Myc PCP Assay positive, while 9 non-PCP patients were PCR negative. In conclusion, these data provide an early indication of the effectiveness of both the Myc Asp Assay and Myc PCP Assay on the ABI 7300 platform for the detection of either Asp or Pj DNA in BAL from patients with deep fungal infections. |
| Databáze: | OpenAIRE |
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