Targeted 1H NMR metabolomics and immunological phenotyping of human fresh blood and serum samples discriminate between healthy individuals and inflammatory bowel disease patients treated with anti-TNF

Autor: Manuel Barreiro-de Acosta, Juan E. Viñuela-Roldán, Sara Notararigo, Adriano Quiroga, Manuel Martín-Pastor, J. Enrique Domínguez-Muñoz
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
medicine.medical_treatment
Proton Magnetic Resonance Spectroscopy
T-Lymphocytes
Disease
Inflammatory bowel disease
0302 clinical medicine
Crohn Disease
Drug Discovery
Medicine
Genetics (clinical)
0303 health sciences
Remission Induction
Middle Aged
Flow Cytometry
Ulcerative colitis
Cytokine
Phenotype
Treatment Outcome
030220 oncology & carcinogenesis
Metabolome
Molecular Medicine
Cytokines
Tumor necrosis factor alpha
Original Article
Female
medicine.symptom
Adult
Inflammation
Immunophenotyping
03 medical and health sciences
NMR metabolomics
Young Adult
Immune system
Predictive Value of Tests
Humans
Metabolomics
030304 developmental biology
Aged
business.industry
medicine.disease
Molecular medicine
Infliximab
IBD stratification
Case-Control Studies
Immunology
IBD metabolomic profiling
Colitis
Ulcerative
Tumor Necrosis Factor Inhibitors
business
Biomarkers
Transcription Factors
Zdroj: Journal of Molecular Medicine (Berlin, Germany)
ISSN: 1432-1440
0946-2716
Popis: Inflammatory bowel disease is a multifactorial etiology, associated with environmental factors that can trigger both debut and relapses. A high level of tumor necrosis factor-α in the gut is the main consequence of immune system imbalance. The aim of treatment is to restore gut homeostasis. In this study, fresh blood and serum samples were used to identify biomarkers and to discriminate between Crohn’s disease and ulcerative colitis patients under remission treated with anti-TNF. Metabolomics based on Nuclear Magnetic Resonance spectroscopy (NMR) was used to detect unique biomarkers for each class of patients. Blood T lymphocyte repertories were characterized, as well as cytokine and transcription factor profiling, to complement the metabolomics data. Higher levels of homoserine-methionine and isobutyrate were identified as biomarkers of Crohn’s disease with ileocolic localization. For ulcerative colitis, lower levels of creatine-creatinine, proline, and tryptophan were found that reflect a deficit in the absorption of essential amino acids in the gut. T lymphocyte phenotyping and its functional profiling revealed that the overall inflammation was lower in Crohn’s disease patients than in those with ulcerative colitis. These results demonstrated that NMR metabolomics could be introduced as a high-throughput evaluation method in routine clinical practice to stratify both types of patients related to their pathology.Key messagesNMR metabolomics is a non-invasive tool that could be implemented in the normal clinical practice for IBD to assess beneficial effect of the treatment.NMR metabolomics is a useful tool for precision medicine, in order to sew a specific treatment to a specific group of patients.Finding predictors of response to IFX would be desirable to select patients affected by IBD.Immunological status of inflammations correlates with NMR metabolomics biomarkers.
Databáze: OpenAIRE
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