The GraRS regulatory system controls Staphylococcus aureus susceptibility to antimicrobial host defenses
| Autor: | Andreas Peschel, Silvia Herbert, Sascha A. Kristian, Victor Nizet, Friedrich Götz, Dirk Kraus, Arya Khosravi |
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| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Staphylococcus aureus Neutrophils medicine.medical_treatment lcsh:QR1-502 Virulence Kidney Staphylococcal infections medicine.disease_cause Microbiology lcsh:Microbiology Cathelicidin Mice Cathelicidins Genes Regulator Operon medicine Animals Humans Cells Cultured Regulator gene Mice Inbred BALB C Alanine biology Lysine Cell Membrane Cytochromes c Phosphatidylglycerols Staphylococcal Infections medicine.disease Antimicrobial Immunity Innate Teichoic Acids Genes Bacterial Myeloperoxidase biology.protein Female Gene Deletion Research Article Antimicrobial Cationic Peptides |
| Zdroj: | BMC Microbiology, Vol 8, Iss 1, p 85 (2008) BMC Microbiology |
| ISSN: | 1471-2180 |
| DOI: | 10.1186/1471-2180-8-85 |
| Popis: | Background Modification of teichoic acids with D-alanine by the products of the dlt operon protects Gram-positive bacteria against major antimicrobial host defense molecules such as defensins, cathelicidins, myeloperoxidase or phospholipase. The gra RS regulatory genes have recently been implicated in the control of D-alanylation in Staphylococcus aureus. Results To determine the impact of the GraRS regulatory system on resistance to antimicrobial host defense mechanisms and virulence of S. aureus, we compared inactivation of S. aureus SA113 wild type and its isogenic gra RS deletion mutant by the human cathelicidin LL-37 or human neutrophil granulocytes in vitro, and the ability to cause infection in vivo. We show here that gra RS deletion considerably alters bacterial surface charge, increases susceptibility to killing by human neutrophils or the defense peptide LL-37, and attenuates virulence of S. aureus in a mouse infection model. Conclusion Our results indicate that S. aureus can regulate its surface properties in order to overcome innate host defenses. |
| Databáze: | OpenAIRE |
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