Functional Effect of the Mutations Similar to the Cleavage during Platelet Activation at Integrin β3 Cytoplasmic Tail when Expressed in Mouse Platelets
| Autor: | Zheng Ruan, Ji-Chun Yang, Dongya Li, Jiansong Huang, Wei Zhang, Xiaodong Xi, Kesheng Dai, Yulan Zhou, Bing Xiao, Jian-Hua Mao, Zhangbiao Long, Xiong-ying Cui, Lanlan Tao, Xiaofeng Shi, Ping Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Integrins Cytoplasm Platelet Aggregation Physiology Amino Acid Motifs Glycobiology lcsh:Medicine Fibrinogen Biochemistry Animal Cells Medicine and Health Sciences Blood and Lymphatic System Procedures Platelet Post-Translational Modification lcsh:Science Bone Marrow Transplantation Multidisciplinary biology Chinese hamster ovary cell Integrin beta3 Hematology Platelet Glycoprotein GPIIb-IIIa Complex Body Fluids Extracellular Matrix Blood Female Anatomy Cellular Types Cellular Structures and Organelles Sequence Analysis Signal Peptides medicine.drug Research Article Protein Binding Signal Transduction Platelets Blood Platelets Integrin Green Fluorescent Proteins Surgical and Invasive Medical Procedures Research and Analysis Methods Transfection 03 medical and health sciences Platelet Adhesiveness Sequence Motif Analysis Platelet adhesiveness medicine Cell Adhesion Animals Platelet activation Amino Acid Sequence Molecular Biology Techniques Sequencing Techniques Molecular Biology Blood Coagulation Glycoproteins Transplantation Blood Cells lcsh:R Fibrinogen binding Biology and Life Sciences Proteins Cell Biology Platelet Activation Molecular biology Mice Inbred C57BL 030104 developmental biology Immobilized Proteins Retroviridae Solubility Hemorheology Mutation biology.protein Mutant Proteins lcsh:Q Collagens |
| Zdroj: | PLoS ONE, Vol 11, Iss 11, p e0166136 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Previous studies in Chinese hamster ovary cells showed that truncational mutations of β3 at sites of F754 and Y759 mimicking calpain cleavage regulate integrin signaling. The roles of the sequence from F754 to C-terminus and the conservative N756ITY759 motif in platelet function have yet to be elaborated. Mice expressing β3 with F754 and Y759 truncations, or NITY deletion (β3-ΔTNITYRGT, β3-ΔRGT, or β3-ΔNITY) were established through transplanting the homozygous β3-deficient mouse bone marrow cells infected by the GFP tagged MSCV MigR1 retroviral vector encoding different β3 mutants into lethally radiated wild-type mice. The platelets were harvested for soluble fibrinogen binding and platelet spreading on immobilized fibrinogen. Platelet adhesion on fibrinogen- and collagen-coated surface under flow was also tested to assess the ability of the platelets to resist hydrodynamic drag forces. Data showed a drastic inhibition of the β3-ΔTNITYRGT platelets to bind soluble fibrinogen and spread on immobilized fibrinogen in contrast to a partially impaired fibrinogen binding and an almost unaffected spreading exhibited in the β3-ΔNITY platelets. Behaviors of the β3-ΔRGT platelets were consistent with the previous observations in the β3-ΔRGT knock-in platelets. The adhesion impairment of platelets with the β3 mutants under flow was in different orders of magnitude shown as: β3-ΔTNITYRGT>β3-ΔRGT>β3-ΔNITY to fibrinogen-coated surface, and β3-ΔTNITYRGT>β3-ΔNITY>β3-ΔRGT to collagen-coated surface. To evaluate the interaction of the β3 mutants with signaling molecules, GST pull-down and immunofluorescent assays were performed. Results showed that β3-ΔRGT interacted with kindlin but not c-Src, β3-ΔNITY interacted with c-Src but not kindlin, while β3-ΔTNITYRGT did not interact with both proteins. This study provided evidence in platelets at both static and flow conditions that the calpain cleavage-related sequences of integrin β3, i.e. T755NITYRGT762, R760GT762, and N756ITY759 participate in bidirectional, outside-in, and inside-out signaling, respectively and the association of c-Src or kindlin with β3 integrin may regulate these processes. |
| Databáze: | OpenAIRE |
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