Xylem systems genetics analysis reveals a key regulator of lignin biosynthesis in Populus deltoides
Autor: | Henry W. Schmidt, Kelly M. Balmant, William B. Barbazuk, Jerald D. Noble, Matias Kirst, Daniel Conde, Gustavo de los Campos, Ana I. Vazquez, Marcio F. R. Resende, Christopher Dervinis, Filipe Couto Alves |
---|---|
Rok vydání: | 2020 |
Předmět: |
Quantitative Trait Loci
Population Biology Lignin Polymorphism Single Nucleotide Genetic analysis Genome Transcriptome 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Wall Gene Expression Regulation Plant Xylem Genetics education Gene Transcription factor Genetics (clinical) 030304 developmental biology 0303 health sciences education.field_of_study Sequence Analysis RNA Research Gene Expression Profiling fungi food and beverages Plants Genetically Modified Phenotype Populus chemistry Genome Plant 030217 neurology & neurosurgery Transcription Factors |
Zdroj: | Genome Res |
ISSN: | 1549-5469 1088-9051 |
Popis: | Despite the growing resources and tools for high-throughput characterization and analysis of genomic information, the discovery of the genetic elements that regulate complex traits remains a challenge. Systems genetics is an emerging field that aims to understand the flow of biological information that underlies complex traits from genotype to phenotype. In this study, we used a systems genetics approach to identify and evaluate regulators of the lignin biosynthesis pathway in Populus deltoides by combining genome, transcriptome, and phenotype data from a population of 268 unrelated individuals of P. deltoides. The discovery of lignin regulators began with the quantitative genetic analysis of the xylem transcriptome and resulted in the detection of 6706 and 4628 significant local- and distant-eQTL associations, respectively. Among the locally regulated genes, we identified the R2R3-MYB transcription factor MYB125 (Potri.003G114100) as a putative trans-regulator of the majority of genes in the lignin biosynthesis pathway. The expression of MYB125 in a diverse population positively correlated with lignin content. Furthermore, overexpression of MYB125 in transgenic poplar resulted in increased lignin content, as well as altered expression of genes in the lignin biosynthesis pathway. Altogether, our findings indicate that MYB125 is involved in the control of a transcriptional coexpression network of lignin biosynthesis genes during secondary cell wall formation in P. deltoides. |
Databáze: | OpenAIRE |
Externí odkaz: |