Intermediate filament-membrane attachments function synergistically with actin-dependent contacts to regulate intercellular adhesive strength
| Autor: | Arthur C. Huen, Xuejun Chen, Kathleen J. Green, Jung K. Park, Irene M. Leigh, Lisa M. Godsel, Leslie J. Bannon, Barry M. Gumbiner, Evangeline V. Amargo, David P. Kelsell, Anne K. Mongiu, Tracie Y. Hudson |
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| Rok vydání: | 2002 |
| Předmět: |
Keratinocytes
DNA Complementary Time Factors Detergents Green Fluorescent Proteins Intermediate Filaments Biology Transfection Article Cell Line Adherens junction Cell membrane 03 medical and health sciences Desmosome Keratoderma Palmoplantar medicine Cell Adhesion Tumor Cells Cultured Humans Biotinylation Cell adhesion Intermediate filament Cytoskeleton 030304 developmental biology 0303 health sciences L-Lactate Dehydrogenase desmosome desmoplakin intermediate filaments cadherins adherens junction Cadherin 030302 biochemistry & molecular biology Cell Membrane Cell Biology Desmosomes Actin cytoskeleton Cadherins Actins Cell biology Protein Structure Tertiary Actin Cytoskeleton Cytoskeletal Proteins Luminescent Proteins medicine.anatomical_structure Desmoplakins Microscopy Fluorescence Desmogleins Protein Binding |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 0021-9525 |
| Popis: | By tethering intermediate filaments (IFs) to sites of intercellular adhesion, desmosomes facilitate formation of a supercellular scaffold that imparts mechanical strength to a tissue. However, the role IF–membrane attachments play in strengthening adhesion has not been directly examined. To address this question, we generated Tet-On A431 cells inducibly expressing a desmoplakin (DP) mutant lacking the rod and IF-binding domains (DPNTP). DPNTP localized to the plasma membrane and led to dissociation of IFs from the junctional plaque, without altering total or cell surface distribution of adherens junction or desmosomal proteins. However, a specific decrease in the detergent-insoluble pool of desmoglein suggested a reduced association with the IF cytoskeleton. DPNTP-expressing cell aggregates in suspension or substrate-released cell sheets readily dissociated when subjected to mechanical stress whereas controls remained largely intact. Dissociation occurred without lactate dehydrogenase release, suggesting that loss of tissue integrity was due to reduced adhesion rather than increased cytolysis. JD-1 cells from a patient with a DP COOH-terminal truncation were also more weakly adherent compared with normal keratinocytes. When used in combination with DPNTP, latrunculin A, which disassembles actin filaments and disrupts adherens junctions, led to dissociation up to an order of magnitude greater than either treatment alone. These data provide direct in vitro evidence that IF–membrane attachments regulate adhesive strength and suggest furthermore that actin- and IF-based junctions act synergistically to strengthen adhesion. |
| Databáze: | OpenAIRE |
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