Autologous fibroblasts induce fibrosis of the nucleus pulposus to maintain the stability of degenerative intervertebral discs
| Autor: | Li Xunlin, Yifan Zhou, Changqing Zhao, Xiao Yang, Zhiqian Chen, Kai Zhang, Jie Zhao, Chen Han, Chen Chen, Haijun Tian, Tangjun Zhou, Xiaojiang Sun, An Qin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases Pathology medicine.medical_specialty Histology Physiology Endocrinology Diabetes and Metabolism Degeneration (medical) Article lcsh:Physiology 03 medical and health sciences 0302 clinical medicine Lumbar Fibrosis medicine Fibroblast Bone lcsh:QH301-705.5 lcsh:QP1-981 business.industry Biomechanics Intervertebral disc medicine.disease musculoskeletal system Low back pain Vertebra Bone quality and biomechanics 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Bone Research, Vol 8, Iss 1, Pp 1-13 (2020) Bone Research |
| ISSN: | 2095-6231 |
| Popis: | Lumbar degenerative disc diseases cause low back pain (LBP). The maintenance of the height and stability of the intervertebral disc (IVD) space is an effective treatment for LBP. The following study evaluated the effects of fibroblast injection on intervertebral disc degeneration (IDD) in a preclinical setting. Compared with the IDD group, the fibroblast treatment group demonstrated effective maintenance of IVD height, reduced endplate degeneration, and improved nuclear magnetic resonance signals and overall histological structure. In doing so, fibrotic IVDs maintained the stability and biomechanics of the vertebra. This finding is in agreement with clinical findings that human nucleus pulposus (NP) fibrosis is essential for the maintenance of IVD height and mechanical properties in patients following percutaneous endoscopic lumbar discectomy (PELD). Mechanistically, we demonstrated that injected fibroblasts not only proliferated but also induced NP cells to adopt a fibrotic phenotype via the secretion of TGF-β. Finally, to better mimic human conditions, the efficacy of autologous fibroblast injection in the treatment of IDD was further examined in a nonhuman primate cynomolgus monkey model due to their capacity for upright posture. We showed that the injection of fibroblasts could maintain the IVD height and rescue IVD signals in cynomolgus monkeys. Taken together, the results of our study reveal that autologous fibroblast injection can enhance the natural process of fibrosis during acute and subacute stages of stress-induced IDD. Fibrotic IVDs can maintain the stability, biological activity, and mechanical properties of the intervertebral space, thus providing a new direction for the treatment of intervertebral space-derived lumbar degenerative diseases. |
| Databáze: | OpenAIRE |
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