Fibrillar vs crystalline nanocellulose pulmonary epithelial cell responses: Cytotoxicity or inflammation?
| Autor: | Ivo Iavicoli, Maria Francesca Russo, Sherri Friend, Galina V. Shurin, Autumn L. Menas, Naveena Yanamala, Mariana T. Farcas, Alexander Star, Philip M. Fournier, Donald H. Beezhold, Bengt Fadeel, Elena R. Kisin, Anna A. Shvedova, Ulla Vogel |
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| Přispěvatelé: | Menas, Autumn L, Yanamala, Naveena, Farcas, Mariana T, Russo, Maria, Friend, Sherri, Fournier, Philip M, Star, Alexander, Iavicoli, Ivo, Shurin, Galina V, Vogel, Ulla B, Fadeel, Bengt, Beezhold, Donald, Kisin, Elena R, Shvedova, Anna A. |
| Rok vydání: | 2017 |
| Předmět: |
Chemokine
Environmental Engineering Cell Survival Health Toxicology and Mutagenesis Lung epithelial cell Nanofibers Inflammation 02 engineering and technology 010501 environmental sciences Cytokine production 01 natural sciences Article Nanocellulose Proinflammatory cytokine chemistry.chemical_compound Cytoxicity Chitin medicine Humans Environmental Chemistry Cellulose Cytotoxicity Lung 0105 earth and related environmental sciences A549 cell biology Public Health Environmental and Occupational Health Epithelial Cells General Medicine General Chemistry 021001 nanoscience & nanotechnology Pollution chemistry Oxidative stress A549 Cells Immunology biology.protein Biophysics Cytokines Nanoparticles Cytokine secretion medicine.symptom 0210 nano-technology |
| Zdroj: | Chemosphere. 171:671-680 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2016.12.105 |
| Popis: | Nanocellulose (NC) is emerging as a highly promising nanomaterial for a wide range of applications. Moreover, many types of NC are produced, each exhibiting a slightly different shape, size, and chemistry. The main objective of this study was to compare cytotoxic effects of cellulose nanocrystals (CNC) and nanofibrillated cellulose (NCF). The human lung epithelial cells (A549) were exposed for 24 h and 72 h to five different NC particles to determine how variations in properties contribute to cellular outcomes, including cytotoxicity, oxidative stress, and cytokine secretion. Our results showed that NCF were more toxic compared to CNC particles with respect to cytotoxicity and oxidative stress responses. However, exposure to CNC caused an inflammatory response with significantly elevated inflammatory cytokines/chemokines compared to NCF. Interestingly, cellulose staining indicated that CNC particles, but not NCF, were taken up by the cells. Furthermore, clustering analysis of the inflammatory cytokines revealed a similarity of NCF to the carbon nanofibers response and CNC to the chitin, a known immune modulator and innate cell activator. Taken together, the present study has revealed distinct differences between fibrillar and crystalline nanocellulose and demonstrated that physicochemical properties of NC are critical in determining their toxicity. |
| Databáze: | OpenAIRE |
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