Enhanced ROS scavenging and sugar accumulation contribute to drought tolerance of naturally occurring autotetraploids in Poncirus trifoliata
| Autor: | Tonglu Wei, Chuanwu Chen, Zongzhou Xie, Ji-Hong Liu, Deng Xiaodong, Dayong Guo, Yue Wang, Qijun Fan |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antioxidant medicine.medical_treatment Drought tolerance drought tolerance Plant Science Biology Poncirus trifoliata 01 natural sciences Transcriptome Superoxide dismutase 03 medical and health sciences Gene Expression Regulation Plant Stress Physiological medicine sugar metabolism Poncirus autotetrploid Sugar Research Articles Plant Proteins chemistry.chemical_classification Reactive oxygen species fungi food and beverages ROS biology.organism_classification Plants Genetically Modified Trifoliate orange Droughts Tetraploidy tetraploid 030104 developmental biology Invertase chemistry Biochemistry biology.protein RNA‐seq Reactive Oxygen Species Sugars Agronomy and Crop Science 010606 plant biology & botany Biotechnology Research Article |
| Zdroj: | Plant Biotechnology Journal |
| ISSN: | 1467-7652 |
| Popis: | Summary Tetraploids have been reported to exhibit increased stress tolerance, but the underlying molecular and physiological mechanisms remain poorly understood. In this study, autotetraploid plants were identified by screening natural seedlings of trifoliate orange (Poncirus trifoliata). The tetraploids exhibited different morphology and displayed significantly enhanced drought and dehydration tolerance in comparison with the diploid progenitor. Transcriptome analysis indicated that a number of stress‐responsive genes and pathways were differentially influenced and enriched in the tetraploids, in particular those coding for enzymes related to antioxidant process and sugar metabolism. Transcript levels and activities of antioxidant enzymes (peroxidase and superoxide dismutase) and sucrose‐hydrolysing enzyme (vacuolar invertase) were increased in the tetraploids upon exposure to the drought, concomitant with greater levels of glucose but lower level of reactive oxygen species (ROS). These data indicate that the tetraploids might undergo extensive transcriptome reprogramming of genes involved in ROS scavenging and sugar metabolism, which contributes, synergistically or independently, to the enhanced stress tolerance of the tetraploid. Our results reveal that the tetraploids take priority over the diploid for stress tolerance by maintaining a more robust system of ROS detoxification and osmotic adjustment via elevating antioxidant capacity and sugar accumulation in comparison with the diploid counterpart. |
| Databáze: | OpenAIRE |
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