A soluble acid invertase is directed to the vacuole by a signal anchor mechanism
| Autor: | Jai M. Perroux, Mark A. Jackson, Rosanne E. Casu, Christopher P. L. Grof, Anne L. Rae |
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| Rok vydání: | 2011 |
| Předmět: |
Cytoplasm
Sucrose Physiology Molecular Sequence Data Plant Science Vacuole Biology Green fluorescent protein chemistry.chemical_compound Saccharum officinarum Sequence Analysis Protein Amino Acid Sequence Tyrosine Peptide sequence Hexoses Plant Proteins Cell Nucleus beta-Fructofuranosidase Membrane Proteins biology.organism_classification Saccharum Protein Transport Invertase chemistry Biochemistry Membrane protein Vacuoles Agronomy and Crop Science |
| Zdroj: | Journal of Plant Physiology. 168:983-989 |
| ISSN: | 0176-1617 |
| DOI: | 10.1016/j.jplph.2010.11.003 |
| Popis: | Enzyme activities in the vacuole have an important impact on the net concentration of sucrose. In sugarcane (Saccharum hybrid), immunolabelling demonstrated that a soluble acid invertase (β-fructofuranosidase; EC 3.2.1.26) is present in the vacuole of storage parenchyma cells during sucrose accumulation. Examination of sequences from sugarcane, barley and rice showed that the N-terminus of the invertase sequence contains a signal anchor and a tyrosine motif, characteristic of single-pass membrane proteins destined for lysosomal compartments. The N-terminal peptide from the barley invertase was shown to be capable of directing the green fluorescent protein to the vacuole in sugarcane cells. The results suggest that soluble acid invertase is sorted to the vacuole in a membrane-bound form. |
| Databáze: | OpenAIRE |
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