Expression of soluble versatile peroxidase of Bjerkandera adusta in Escherichia coli
| Autor: | Roman Jerala, Martina Mohorčič, Mojca Benčina, Jožefa Friedrich |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Environmental Engineering
Bioengineering medicine.disease_cause Microbiology Substrate Specificity Bjerkandera adusta Manganese peroxidase Enzyme Stability medicine Escherichia coli Versatile peroxidase Waste Management and Disposal Peroxidase biology Renewable Energy Sustainability and the Environment General Medicine Lignin peroxidase biology.organism_classification Recombinant Proteins Enzyme Activation Biochemistry Solubility Enzyme model biology.protein Heterologous expression Coriolaceae |
| Zdroj: | Bioresource technology. 100(2) |
| ISSN: | 1873-2976 |
| Popis: | Versatile peroxidase from white rot fungus Bjerkandera adusta was over-expressed in a soluble form in Escherichia coli . In the constructed enzyme model based on the selected gene from B. adusta , the active sites for oxidation of Mn 2+ ions and for oxidation of aromatic substrates were identified, both characteristic for versatile peroxidase. For over-expression of the recombinant enzyme different host strains, media formulations, growth temperatures, and fusion partners were tested. With the bacterial strain BL21(DE3)pLysS cultivated at 25 °C in auto-induction medium and presence of heme, a soluble peroxidase with incorporated heme and activity against different substrates was obtained. By exploiting an appropriate expression system and providing suitable culture conditions, the recombinant fungal peroxidases in soluble form can be produced in bacteria. |
| Databáze: | OpenAIRE |
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