TaCYP81D5, one member in a wheat cytochrome P450 gene cluster, confers salinity tolerance via reactive oxygen species scavenging
Autor: | Weiming Shi, Guangmin Xia, Shuwei Liu, Jiarui Yuan, Lumin Qin, Meng Wang |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine reactive oxygen species scavenging Triticum aestivum Plant Science 01 natural sciences salinity tolerance 03 medical and health sciences Cytochrome P-450 Enzyme System Gene Expression Regulation Plant Stress Physiological Zat12 Gene cluster Gene family Prospective Studies Gene Research Articles Triticum Plant Proteins chemistry.chemical_classification Reactive oxygen species Gene knockdown biology Cytochrome P450 food and beverages Salt Tolerance biology.organism_classification Cell biology Salinity 030104 developmental biology TaCYP81D5 chemistry Multigene Family biology.protein Brachypodium distachyon Reactive Oxygen Species Agronomy and Crop Science 010606 plant biology & botany Biotechnology Research Article |
Zdroj: | Plant Biotechnology Journal |
ISSN: | 1467-7652 1467-7644 |
Popis: | Summary As one of the largest gene families in plants, the cytochrome P450 monooxygenase genes (CYPs) are involved in diverse biological processes including biotic and abiotic stress response. Moreover, P450 genes are prone to expanding due to gene tandem duplication during evolution, resulting in generations of novel alleles with the neo‐function or enhanced function. Here, the bread wheat (Triticum aestivum) gene TaCYP81D5 was found to lie within a cluster of five tandemly arranged CYP81D genes, although only a single such gene (BdCYP81D1) was present in the equivalent genomic region of the wheat relative Brachypodium distachyon. The imposition of salinity stress could up‐regulate TaCYP81D5, but the effect was abolished in plants treated with an inhibitor of reactive oxygen species synthesis. In SR3, a wheat cultivar with an elevated ROS content, the higher expression and the rapider response to salinity of TaCYP81D5 were related to the chromatin modification. Constitutively expressing TaCYP81D5 enhanced the salinity tolerance both at seedling and reproductive stages of wheat via accelerating ROS scavenging. Moreover, an important component of ROS signal transduction, Zat12, was proven crucial in this process. Though knockout of solely TaCYP81D5 showed no effect on salinity tolerance, knockdown of BdCYP81D1 or all TaCYP81D members in the cluster caused the sensitivity to salt stress. Our results provide the direct evidence that TaCYP81D5 confers salinity tolerance in bread wheat and this gene is prospective for crop improvement. |
Databáze: | OpenAIRE |
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