Caspase inhibitors affect the kinetics and dimensions of tracheary elements in xylogenic Zinnia (Zinnia elegans) cell cultures
| Autor: | Wim van Ieperen, Ernst J. Woltering, Anne Mie C. Emons, Peter Twumasi, Tian Qian, Olaf van Kooten, J.H.N. Schel, Elena T. Iakimova |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Hypersensitive response
Proteases Programmed cell death serine proteases hypersensitive response tomato suspension cells Cellular differentiation Cell Culture Techniques Leerstoelgroep Tuinbouwproductieketens plant caspases Plant Science Asteraceae dna fragmentation vascular development Xylem lcsh:Botany death FBR Fresh Supply Chains Enzyme Inhibitors Caspase mesophyll-cells Horticultural Supply Chains biology fungi Zinnia elegans food and beverages Cell Differentiation Laboratorium voor Celbiologie differentiation biology.organism_classification PE&RC lcsh:QK1-989 Cell biology Zinnia Laboratory of Cell Biology Cell culture biology.protein nuclear-dna Research Article |
| Zdroj: | BMC Plant Biology BMC Plant Biology, 10 BMC Plant Biology, Vol 10, Iss 1, p 162 (2010) BMC Plant Biology 10 (2010) |
| ISSN: | 1471-2229 |
| DOI: | 10.1186/1471-2229-10-162 |
| Popis: | Background The xylem vascular system is composed of fused dead, hollow cells called tracheary elements (TEs) that originate through trans-differentiation of root and shoot cambium cells. TEs undergo autolysis as they differentiate and mature. The final stage of the formation of TEs in plants is the death of the involved cells, a process showing some similarities to programmed cell death (PCD) in animal systems. Plant proteases with functional similarity to proteases involved in mammalian apoptotic cell death (caspases) are suggested as an integral part of the core mechanism of most PCD responses in plants, but participation of plant caspase-like proteases in TE PCD has not yet been documented. Results Confocal microscopic images revealed the consecutive stages of TE formation in Zinnia cells during trans-differentiation. Application of the caspase inhibitors Z-Asp-CH2-DCB, Ac-YVAD-CMK and Ac-DEVD-CHO affected the kinetics of formation and the dimensions of the TEs resulting in a significant delay of TE formation, production of larger TEs and in elimination of the 'two-wave' pattern of TE production. DNA breakdown and appearance of TUNEL-positive nuclei was observed in xylogenic cultures and this was suppressed in the presence of caspase inhibitors. Conclusions To the best of our knowledge this is the first report showing that caspase inhibitors can modulate the process of trans-differentiation in Zinnia xylogenic cell cultures. As caspase inhibitors are closely associated with cell death inhibition in a variety of plant systems, this suggests that the altered TE formation results from suppression of PCD. The findings presented here are a first step towards the use of appropriate PCD signalling modulators or related molecular genetic strategies to improve the hydraulic properties of xylem vessels in favour of the quality and shelf life of plants or plant parts. |
| Databáze: | OpenAIRE |
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