The Conserved RNA Exonuclease Rexo5 Is Required for 3′ End Maturation of 28S rRNA, 5S rRNA, and snoRNAs
| Autor: | Hermann Steller, Joe Rodriguez, Cindy Meyer, Daniel Briskin, Sigi Benjamin-Hong, Stefanie Gerstberger, Thomas Tuschl, Claudia Bognanni, Kimberly Bogardus |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Exonuclease Mitosis Ribosome Article General Biochemistry Genetics and Molecular Biology U8 snoRNA 03 medical and health sciences 5S ribosomal RNA RNA exonuclease SnoRNA processing RNA Ribosomal 28S Animals Drosophila Proteins RNA Small Nucleolar rRNA 3′ end maturation RNA Processing Post-Transcriptional Small nucleolar RNA Gonads RRNA processing lcsh:QH301-705.5 Conserved Sequence rRNA processing Genetics biology urogenital system Homozygote snoRNA biogenesis RNA Ribosomal RNA Drosophila melanogaster 030104 developmental biology rRNA biogenesis lcsh:Biology (General) Exoribonucleases Mutation biology.protein snoRNA 3′ end maturation Female snoRNA processing Ribosomes |
| Zdroj: | Cell Reports, Vol 21, Iss 3, Pp 758-772 (2017) |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2017.09.067 |
| Popis: | Non-coding RNA biogenesis in higher eukaryotes has not been fully characterized. Here, we studied the Drosophila melanogaster Rexo5 (CG8368) protein, a metazoan-specific member of the DEDDh 3′-5′ single-stranded RNA exonucleases, by genetic, biochemical, and RNA sequencing approaches. Rexo5 is required for small nucleolar RNA (snoRNA) and ribosomal RNA (rRNA) biogenesis, and is essential in D. melanogaster. Loss-of-function mutants accumulate improperly 3′-end-trimmed 28S rRNA, 5S rRNA, and snoRNA precursors in vivo. Rexo5 is ubiquitously expressed at low levels in somatic metazoan cells, but extremely elevated in male and female germ cells. Loss of Rexo5 leads to increased nucleolar size, genomic instability, defective ribosome subunit export and larval death. Loss-of-germline expression compromises gonadal growth and meiotic entry during germline development. |
| Databáze: | OpenAIRE |
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