Diamond nanopillar arrays for quantum microscopy of neuronal signals
| Autor: | Prithvi Reddy, Marika Niihori, Marcus W. Doherty, James D. A. Wood, Jörg Wrachtrup, Matthew Sellars, Gregory J Stuart, Liam Hanlon, Ben Corry, Alexander R. J. Silalahi, Vini Gautam, Patrick Maletinsky, Michael S. J. Barson, Vincent Ricardo Daria |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Paper
Materials science Field (physics) Neuroscience (miscellaneous) nanopillars neurons 01 natural sciences 010309 optics nitrogen-vacancy 03 medical and health sciences 0302 clinical medicine Electric field 0103 physical sciences Microscopy Radiology Nuclear Medicine and imaging Quantum Nanopillar neuroimaging Radiological and Ultrasound Technology business.industry Quantum sensor neuromodeling Research Papers Magnetic field Optoelectronics business 030217 neurology & neurosurgery Excitation |
| Zdroj: | Neurophotonics |
| ISSN: | 2329-4248 2329-423X |
| Popis: | Significance: Wide-field measurement of cellular membrane dynamics with high spatiotemporal resolution can facilitate analysis of the computing properties of neuronal circuits. Quantum microscopy using a nitrogen-vacancy (NV) center is a promising technique to achieve this goal. Aim: We propose a proof-of-principle approach to NV-based neuron functional imaging. Approach: This goal is achieved by engineering NV quantum sensors in diamond nanopillar arrays and switching their sensing mode to detect the changes in the electric fields instead of the magnetic fields, which has the potential to greatly improve signal detection. Apart from containing the NV quantum sensors, nanopillars also function as waveguides, delivering the excitation/emission light to improve sensitivity. The nanopillars also improve the amplitude of the neuron electric field sensed by the NV by removing screening charges. When the nanopillar array is used as a cell niche, it acts as a cell scaffolds which makes the pillars function as biomechanical cues that facilitate the growth and formation of neuronal circuits. Based on these growth patterns, numerical modeling of the nanoelectromagnetics between the nanopillar and the neuron was also performed. Results: The growth study showed that nanopillars with a 2-μm pitch and a 200-nm diameter show ideal growth patterns for nanopillar sensing. The modeling showed an electric field amplitude as high as ≈1.02×1010 mV/m at an NV 100 nm from the membrane, a value almost 10 times the minimum field that the NV can detect. Conclusion: This proof-of-concept study demonstrated unprecedented NV sensing potential for the functional imaging of mammalian neuron signals. |
| Databáze: | OpenAIRE |
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