Redirection of auxin flow in Arabidopsis thaliana roots after infection by root-knot nematodes

Autor: Janice de Almeida Engler, S. Warmerdam, Aska Goverse, Gilbert Engler, Annelies Haegeman, Mona Jahani, Cecilia Ngonyo Wanjau, Godelieve Gheysen, Tina Kyndt
Přispěvatelé: Department of Molecular Biotechnology, State University of Ghent, Wageningen University and Research Centre (WUR), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), COST Actions [872, FA1208, COST-STSM-872-05044], Dutch Technology Foundation STW part of the Netherlands Organisation for Scientific Research (NWO) - Ministry of Economic Affairs, FWO postdoctoral fellowship
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
root-knot nematode
EFFLUX
Physiology
[SDV]Life Sciences [q-bio]
Mutant
Arabidopsis
Plant Science
Plant Roots
01 natural sciences
Plant Growth Regulators
Gall
heterocyclic compounds
chemistry.chemical_classification
Microscopy
Confocal
biology
Membrane transport protein
INDUCTION
food and beverages
LOCALIZATION
ETHYLENE
[SDE]Environmental Sciences
galls
Research Paper
Auxin influx
DIRECTS AUXIN
Gravitropism
PLANT-PARASITIC NEMATODES
GRADIENTS
GRAVITROPISM
03 medical and health sciences
CYST
Auxin
Botany
Animals
Root-knot nematode
Tylenchoidea
Laboratorium voor Nematologie
Indoleacetic Acids
Arabidopsis Proteins
FEEDING SITES
fungi
AUX1
Membrane Transport Proteins
Biology and Life Sciences
PIN
biology.organism_classification
LAX3
030104 developmental biology
chemistry
biology.protein
EPS
Laboratory of Nematology
auxin
010606 plant biology & botany
Zdroj: Journal of Experimental Botany
Journal of Experimental Botany, Oxford University Press (OUP), 2016, 67 (15), pp.4559-4570. ⟨10.1093/jxb/erw230⟩
JOURNAL OF EXPERIMENTAL BOTANY
Journal of Experimental Botany, 67(15), 4559-4570
Journal of Experimental Botany 67 (2016) 15
ISSN: 0022-0957
1460-2431
4559-4570
DOI: 10.1093/jxb/erw230⟩
Popis: Highlight Plant auxin efflux and influx proteins redirect the plant hormone auxin towards the feeding site upon root-knot nematode infection in Arabidopsis thaliana roots.
Plant-parasitic root-knot nematodes induce the formation of giant cells within the plant root, and it has been recognized that auxin accumulates in these feeding sites. Here, we studied the role of the auxin transport system governed by AUX1/LAX3 influx proteins and different PIN efflux proteins during feeding site development in Arabidopsis thaliana roots. Data generated via promoter–reporter line and protein localization analyses evoke a model in which auxin is being imported at the basipetal side of the feeding site by the concerted action of the influx proteins AUX1 and LAX3, and the efflux protein PIN3. Mutants in auxin influx proteins AUX1 and LAX3 bear significantly fewer and smaller galls, revealing that auxin import into the feeding sites is needed for their development and expansion. The feeding site development in auxin export (PIN) mutants was only slightly hampered. Expression of some PINs appears to be suppressed in galls, probably to prevent auxin drainage. Nevertheless, a functional PIN4 gene seems to be a prerequisite for proper nematode development and gall expansion, most likely by removing excessive auxin to stabilize the hormone level in the feeding site. Our data also indicate a role of local auxin peaks in nematode attraction towards the root.
Databáze: OpenAIRE
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