What Makes a Weed a Weed? How Virus-mediated Reverse Genetics Can Help to Explore the Genetics of Weediness
| Autor: | Macgregor, D. |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Transgene
fungi Gene regulatory network food and beverages Computational biology Biology Weed Molecular Biology Reverse Genetics Reverse genetics Virus Virus-Mediated Reverse Genetics (VMRG) Virus-Induced Gene Silencing (VIGS) Virus Mediated Overexpression (VOX) Insect Science Gene silencing Weed Transient Transformation Agronomy and Crop Science Gene Function (biology) Herbicide Resistance Food Science Biotechnology |
| Zdroj: | Outlooks on Pest Management. 31:224-229 |
| ISSN: | 1743-1026 |
| DOI: | 10.1564/v31_oct_07 |
| Popis: | Reverse genetics investigates what a gene does by testing how the plant responds when the specific gene is changed. These techniques have been in use for decades to assess whether a given gene underpins interesting phenotypes and gain insight into the function of gene networks and families. Weed science has only recently entered the "genomic era" in which genomic and reverse genetics approaches are used to address hypotheses. This review focuses on two reverse genetic techniques used on a variety of plants including agricultural weeds, virus-induced gene silencing (VIGS) and virus-mediated overexpression (VOX), explaining the biology behind them and highlighting how these tools may be used for gene function validation in weed species for which no other transgenic approaches have been developed. |
| Databáze: | OpenAIRE |
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