Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii
| Autor: | Heloisa D'Avila, Sergio Henrique Seabra, Laura Azeredo Miranda Mota, Marco Antonio de Oliveira, V.G. Monteiro, Caroliny Samary Silva Lobato, Maura Da Cunha, Renato Augusto DaMatta, Patrícia T. Bozza, João Roberto Neto |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Male lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Indomethacin Primary Cell Culture lcsh:QR1-502 Toxoplasma gondii Inflammation Vacuole Biology lcsh:Microbiology Microbiology Host-Parasite Interactions Mice Microscopy Electron Transmission nitric oxide Lipid droplet Organelle parasitic diseases medicine Macrophage Animals Mice Inbred C3H Prostaglandins E Lipid metabolism Lipid Droplets Articles Macrophage Activation biology.organism_classification macrophages Cell culture Immunology Vacuoles Macrophages Peritoneal Microscopy Electron Scanning Cattle medicine.symptom prostaglandin lipid bodies Toxoplasma microbicidal capacity |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 109, Iss 6, Pp 767-774 (2014) Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz, Volume: 109, Issue: 6, Pages: 767-774, Published: 13 AUG 2014 |
| ISSN: | 1678-8060 |
| Popis: | Lipid bodies [lipid droplets (LBs)] are lipid-rich organelles involved in lipid metabolism, signalling and inflammation. Recent findings suggest a role for LBs in host response to infection; however, the potential functions of this organelle in Toxoplasma gondii infection and how it alters macrophage microbicidal capacity during infection are not well understood. Here, we investigated the role of host LBs in T. gondii infection in mouse peritoneal macrophages in vitro. Macrophages cultured with mouse serum (MS) had higher numbers of LBs than those cultured in foetal bovine serum and can function as a model to study the role of LBs during intracellular pathogen infection. LBs were found in association with the parasitophorous vacuole, suggesting that T. gondii may benefit from this lipid source. Moreover, increased numbers of macrophage LBs correlated with high prostaglandin E2 (PGE2) production and decreased nitric oxide (NO) synthesis. Accordingly, LB-enriched macrophages cultured with MS were less efficient at controlling T. gondii growth. Treatment of macrophages cultured with MS with indomethacin, an inhibitor of PGE2 production, increased the microbicidal capacity against T. gondii. Collectively, these results suggest that culture with MS caused a decrease in microbicidal activity of macrophages against T. gondii by increasing PGE2 while lowering NO production. |
| Databáze: | OpenAIRE |
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