Involvement of serotonergic and opioidergic systems in the antinociceptive effect of ketamine-magnesium sulphate combination in formalin test in rats
| Autor: | Katarina Savić Vujović, Milica Prostran, Dolika Vasović, Nevena Divac, Branislava Medić, Radan Stojanović, Dragana Srebro, Sonja Vučković |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Methysergide Pain chemistry.chemical_element (+)-Naloxone Pharmacology Serotonergic Magnesium Sulfate 03 medical and health sciences 0302 clinical medicine Formaldehyde medicine Animals Ketamine Rats Wistar Pain Measurement Opioidergic Analgesics Dose-Response Relationship Drug Naloxone Magnesium General Medicine Rats Dose–response relationship Nociception chemistry 030220 oncology & carcinogenesis Drug Therapy Combination 030217 neurology & neurosurgery Serotonergic Neurons medicine.drug |
| Zdroj: | Pharmacological Reports. 71:1014-1019 |
| ISSN: | 1734-1140 |
| Popis: | Background Ketamine and magnesium sulphate showed synergic interaction in the tail-immersion test and additive interaction in the rat formalin test. Aim of study was to evaluate the influence of serotonergic and opioidergic system of this combination in the formalin test in rats. Methods Antinociceptive activity was assessed by the formalin test in male Wistar rats (200–250 g). Antagonists (naloxone and methysergide) were administrated 5 min before and magnesium sulphate 5 min after ketamine injection. Formalin (2.5%, 100 μL) was injected into the right hind paw surface (intraplantar) of rats 5 min after ketamine/magnesium combination. Data were recorded as the total time spent in pain related behavior after the injection of formalin or vehicle (0.9% NaCl). Results In the intermediate phase of the formalin test, methysergide at a dose of 0.2 mg/kg did not have any effect, but at doses of 0.5 and 1 mg/kg it had a pronociceptive effect. Methysergide (0.2, 0.5 and 1 mg/kg) inhibited the antinociceptive effect of ketamine-magnesium sulphate combination. In the intermediate phase, naloxone at a dose of 0.2 mg/kg did not have any effect, but at a dose of 3 mg/kg it produced a pronociceptive effect. Naloxone (0.2 and 3 mg/kg) antagonized the antinociceptive effect of the ketamine (5 mg/kg)-magnesium sulphate (5 mg/kg) combination. Conclusion The results of the present study suggest that serotonergic and opioidergic systems are involved, at least in part, in the antinociceptive effect of the ketamine-magnesium sulphate combination in the model of inflammatory pain in rats. |
| Databáze: | OpenAIRE |
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