Dynamic single-cell intracellular pH sensing using a SERS-active nanopipette
| Autor: | Joseph Moscoso, Alberto Sesena Rubfiaro, Jing Guo, Yuan Liu, Xuewen Wang, Jin He, Yanhao Lai, Feng Chen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Intracellular pH
Cell Nanoparticle Metal Nanoparticles 02 engineering and technology 010402 general chemistry Spectrum Analysis Raman 01 natural sciences Biochemistry Article Analytical Chemistry HeLa symbols.namesake Electrochemistry medicine Extracellular Environmental Chemistry Humans Spectroscopy Plasmon biology Chemistry Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences medicine.anatomical_structure symbols Biophysics Gold 0210 nano-technology Raman spectroscopy Intracellular HeLa Cells |
| Zdroj: | Analyst |
| ISSN: | 1364-5528 |
| Popis: | Glass nanopipette has shown promise in applications for single-cell manipulation, analysis, and imaging. In recent years, plasmonic nanopipette has been developed for single-cell analysis to take the advantage of surface-enhanced Raman spectroscopy (SERS) measurement. In this work, we developed a SERS-active nanopipette that can perform long-term and reliable intracellular analysis of single living cell with minimal damage, which is achieved by optimizing the nanopipette geometry and the surface density of gold nanoparticle (AuNP) layer at the nanopipette tip. To demonstrate its capability in single-cell analysis, we have applied the nanopipette for intracellular pH sensing. Intracellular pH (pH(i)) is vital to cells as it influences cell functions, behaviors and pathological conditions. The pH-sensitivity was realized by simply modifying the AuNP layer with pH reporter molecule 4-Mercaptobenzoic acid. With a response time of less than 5 seconds, the pH sensing range is from 6.0 to 8.0 and the maximum sensitivity is 0.2 pH units. We have monitored the pH(i) change of individual HeLa and Fibroblast cells, triggered by the extracellular pH (pH(e)) change. The HeLa cancer cell can better resist pH(e) change and adapt to the weak acidic environment. The plasmonic nanopipette can be further developed to monitor other intracellular biomarkers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|