Genomic diversity of Bartonella henselae isolates from domestic cats from Japan, the USA and France by pulsed-field gel electrophoresis
| Autor: | Henri Jean Boulouis, Bruno B Chomel, Nikos A. Gurfield, Soichi Maruyama, Rickie W. Kasten, Yasuji Katsube |
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| Rok vydání: | 2001 |
| Předmět: |
DNA
Bacterial Bartonellaceae Cat Diseases Polymerase Chain Reaction Microbiology Bartonella clarridgeiae Japan Pulsed-field gel electrophoresis Animals Cluster Analysis Phylogeny Gel electrophoresis Genetics Bartonella henselae CATS General Veterinary biology Cat-Scratch Disease Genetic Variation General Medicine biology.organism_classification United States Electrophoresis Gel Pulsed-Field Restriction enzyme genomic DNA Cats France Genome Bacterial |
| Zdroj: | Veterinary Microbiology. 79:337-349 |
| ISSN: | 0378-1135 |
| Popis: | The genomic DNA diversity of 27 Bartonella henselae and three B. clarridgeiae isolates from 18 domestic cats from Japan, the USA and France was investigated by pulsed-field gel electrophoresis (PFGE) with Not I, Asc I and Sma I restriction enzymes. A great diversity of genomic patterns was found for all B. henselae , but none for B. clarridgeiae isolates. The DNA size of B. henselae and B. clarridgeiae isolates were 1.7–2.9 and 1.7 Mbp, respectively. All 13 Japanese cat isolates were identified as B. henselae type I. Furthermore, three of the four Japanese cats harbored genetically different B. henselae type I isolates, suggesting for the first time co-infection with various type I isolates. One French cat and one American cat were co-infected with B. henselae and B. clarridgeiae . B. henselae type I and type II were mainly grouped in two different clusters by PFGE using Sma I endonuclease in the dendrogram. |
| Databáze: | OpenAIRE |
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