Dithizone-induced Paneth cell disruption significantly decreases intestinal perfusion in the murine small intestine

Autor: Jennifer N. Berger, Misty Good, Steven J. McElroy, Huyiu Gong
Rok vydání: 2019
Předmět:
Small
Pediatrics
Intestinal microvasculature
Mice
chemistry.chemical_compound
0302 clinical medicine
Necrotizing enterocolitis
Ischemia
Intestine
Small
Diphtheria Toxin
Pediatric
General Medicine
Intestine
medicine.anatomical_structure
Dithizone
030220 oncology & carcinogenesis
medicine.symptom
Perfusion
Biotechnology
Signal Transduction
Paneth Cells
Nitric Oxide
Article
Nitric oxide
Paediatrics and Reproductive Medicine
03 medical and health sciences
Enterocolitis
Necrotizing
030225 pediatrics
medicine
Animals
Animal model
Diphtheria toxin
Enterocolitis
Animal
business.industry
Microcirculation
Perinatal Period - Conditions Originating in Perinatal Period
medicine.disease
Molecular biology
Small intestine
Disease Models
Animal
chemistry
Disease Models
Pediatrics
Perinatology and Child Health
Paneth cell
Surgery
Necrotizing
Digestive Diseases
business
Vasoconstriction
Zdroj: J Pediatr Surg
Journal of pediatric surgery, vol 54, iss 11
ISSN: 0022-3468
DOI: 10.1016/j.jpedsurg.2019.02.021
Popis: PurposeNecrotizing enterocolitis is associated with decreased intestinal perfusion and ischemia. Paneth cells, specialized epithelial cells, have been shown to regulate the intestinal vasculature and disruption of these cells has been associated with NEC. We hypothesized that Paneth cell disruption in immature mice intestine would decrease the perfusion of the intestinal microvasculature.MethodsPaneth cells were disrupted in P14-16 mice using chemical (dithizone) and transgenic (diphtheria toxin) methodology. Six hours after Paneth cell disruption, Dylight 488 was injected directly into the left ventricle and allowed to perfuse for 5 minutes prior to intestinal harvesting. Tissue samples were evaluated with confocal fluorescence microscopy to quantify intestinal perfusion and samples were quantified by real time RT-PCR for gene expression.ResultsDithizone treatment significantly decreased intestinal perfusion compared to controls (p 0.21). Intestines from all treatment groups had similar PECAM staining, but intestines treated with dithizone had significantly decreased nNOS and iNOS gene expression compared to controls (p
Databáze: OpenAIRE
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