In vivo temperature heterogeneity is associated with plaque regions of increased MMP-9 activity
| Autor: | Chaylendra Strijder, Anton F. W. van der Steen, Gerard Pasterkamp, Eric Boersma, Mark M. Kockx, Babak Mousavi Gourabi, Patrick W. Serruys, Glenn Van Langenhove, Rob Krams, Dennie Tempel, Stefan Verheye, Luc C.A. van Damme, Michiel W.M. Knaapen |
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| Přispěvatelé: | Cardiology, Erasmus MC other |
| Rok vydání: | 2005 |
| Předmět: |
Male
Pathology medicine.medical_specialty Hot Temperature Myocytes Smooth Muscle Cell Inflammation Matrix metalloproteinase Muscle Smooth Vascular In vivo Animals Medicine Macrophage Myocyte Zymography Aorta chemistry.chemical_classification business.industry Macrophages Atherosclerosis Molecular biology Cold Temperature medicine.anatomical_structure Enzyme Matrix Metalloproteinase 9 chemistry Thermography Models Animal Rabbits medicine.symptom Cardiology and Cardiovascular Medicine business |
| Zdroj: | European Heart Journal, 26(20), 2200-2205. Oxford University Press |
| ISSN: | 1522-9645 0195-668X |
| Popis: | AIMS: Plaque rupture has been associated with a high matrix metalloproteinase (MMP) activity. Recently, regional temperature variations have been observed in atherosclerotic plaques in vivo and ascribed to the presence of macrophages. As macrophages are a major source of MMPs, we examined whether regional temperature changes are related to local MMP activity and macrophage accumulation. METHODS AND RESULTS: Plaques were experimentally induced in rabbit (n=11) aortas, and at the day of sacrifice, a pull-back was performed with a thermography catheter. Hot (n=10), cold (n=10), and reference (n=11) regions were dissected and analysed for smooth muscle cell (SMC), lipids (L), collagen (COL), and macrophage (MPhi) cell densities (%); a vulnerability index (VI) was calculated as VI=MPhi+L/(SMC+COL). In addition, accumulation and activity of MMP-2 and MMP-9 were determined with zymography. Ten hot regions were identified with an average temperature of 0.40+/-0.03 degrees C (P |
| Databáze: | OpenAIRE |
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