Comparison of the Cepheid Xpert Xpress Flu/RSV Assay to in-house Flu/RSV triplex real-time RT-PCR for rapid molecular detection of Influenza A, Influenza B and Respiratory Syncytial Virus in respiratory specimens
| Autor: | Yolanda I.I. Ho, Ann H. Wong, Raymond Lai |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) viruses 030106 microbiology Influenza epidemics Respiratory Syncytial Virus Infections Real-Time Polymerase Chain Reaction Microbiology Sensitivity and Specificity Virus 03 medical and health sciences Nasopharyngeal aspirate Nasopharynx Influenza Human Medicine Infection control Humans Respiratory system Flocked swab DNA Primers business.industry virus diseases Influenza a General Medicine Virology Respiratory Syncytial Viruses Influenza B virus Real-time polymerase chain reaction Molecular Diagnostic Techniques Influenza A virus Biological Assay business Multiplex Polymerase Chain Reaction |
| Zdroj: | Journal of medical microbiology. 67(11) |
| ISSN: | 1473-5644 |
| Popis: | This study compared the performance of the commercially available Xpert Xpress Flu/RSV assay to an in-house FluAB/RSV triplex real-time RT-PCR assay for the detection of influenza A/B viruses and respiratory syncitial virus (RSV) from both nasopharyngeal aspirate (NPA) and nasopharyngeal flocked swab (NPS). A total of 20 external quality assurance (EQA) samples and 172 clinical respiratory samples were tested prospectively using both the Xpert Xpress Flu/RSV assay and the in-house FluAB/RSV triplex assay. For the EQA samples, concordance rate was 100 % when tested with both assays. For clinical samples, there was 100 % agreement between the two assays for detection of influenza A and influenza B, 96.7 % agreement for detection of RSV and 99.7 % agreement for negative results. With a shortened turnaround time and good diagnostic performance, application of the Xpert Xpress Flu/RSV assay can facilitate patient triage for prompt implementation of infection control measures and management of high-risk patients during influenza epidemics. |
| Databáze: | OpenAIRE |
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