Tear metabolomics highlights new potential biomarkers for differentiating between Sjögren's syndrome and other causes of dry eye
| Autor: | Odile Blanchet, Juan Manuel Chao de la Barca, Delphine Prunier-Mirebeau, Sophie Assad, Floris Chabrun, Christian Lavigne, Gilles Simard, Philippe Gohier, Geoffrey Urbanski, Pascal Reynier, Guy Lenaers |
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| Rok vydání: | 2021 |
| Předmět: |
Oncology
medicine.medical_specialty Population Dry Eye Syndromes chemistry.chemical_compound Metabolomics stomatognathic system Sicca syndrome Internal medicine Humans Medicine Prospective Studies education Prospective cohort study education.field_of_study business.industry eye diseases stomatognathic diseases Ophthalmology Sjogren's Syndrome chemistry Biomarker (medicine) Tears business Biomarkers PCAA |
| Zdroj: | The Ocular Surface. 22:110-116 |
| ISSN: | 1542-0124 |
| DOI: | 10.1016/j.jtos.2021.07.006 |
| Popis: | Purpose The lacrimal exocrinopathy of primary Sjogren's syndrome (pSS) is one of the main causes of severe dry eye syndrome and a burden for patients. Early recognition and treatment could prevent irreversible damage to lacrimal glands. The aim of this study was to find biomarkers in tears, using metabolomics and data mining approaches, in patients with newly-diagnosed pSS compared to other causes of dry eye syndrome. Methods A prospective cohort of 40 pSS and 40 non-pSS Sicca patients with dryness was explored through a standardized targeted metabolomic approach using liquid chromatography coupled with mass spectrometry. A metabolomic signature predictive of the pSS status was sought out using linear (logistic regression with elastic-net regularization) and non-linear (random forests) machine learning architectures, after splitting the studied population into training, validation and test sets. Results Among the 104 metabolites accurately measured in tears, we identified a discriminant signature composed of nine metabolites (two amino acids: serine, aspartate; one biogenic amine: dopamine; six lipids: Lysophosphatidylcholine C16:1, C18:1, C18:2, sphingomyelin C16:0 and C22:3, and the phoshatidylcholine diacyl PCaa C42:4), with robust performances (ROC-AUC = 0.83) for predicting the pSS status. Adjustment for age, sex and anti-SSA antibodies did not disrupt the link between the metabolomic signature and the pSS status. The non-lipidic components also remained specific for pSS regardless of the dryness severity. Conclusion Our results reveal a metabolomic signature for tears that distinguishes pSS from other dry eye syndromes and further highlight nine key metabolites of potential interest for early diagnosis and therapeutics of pSS. |
| Databáze: | OpenAIRE |
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