Ketamine anesthesia helps preserve neuronal viability
| Autor: | Dirk F. van Helden, Mohammad S. Imtiaz, Fernanda S. Gravina, Rebecca Lim, Robert J. Callister, Ramatis B. de Oliveira, Alan M. Brichta, Marcus H. C. Howlett, Max Oliveira, Brett A. Graham |
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| Přispěvatelé: | Netherlands Institute for Neuroscience (NIN) |
| Rok vydání: | 2010 |
| Předmět: |
Hypoglossal Nerve
Patch-Clamp Techniques Cell Survival medicine.drug_class In Vitro Techniques Electric Capacitance Dissociative Membrane Potentials Mice Slice preparation Electric Impedance medicine Animals Anesthesia Ketamine Patch clamp Motor Neurons Neurons Anesthetics Dissociative business.industry General Neuroscience Antagonist Organ Preservation Animals Newborn nervous system Anesthetic Tissue and Organ Harvesting NMDA receptor Locus coeruleus Locus Coeruleus business Neuroscience medicine.drug |
| Zdroj: | Journal of Neuroscience Methods, 189, 230-232. Elsevier B.V. |
| ISSN: | 0165-0270 |
| DOI: | 10.1016/j.jneumeth.2010.03.029 |
| Popis: | The dissociative anesthetic ketamine that acts as an N-methyl-D-aspartate (NMDA) antagonist has been reported to improve neurological damage after experimental ischemic challenges. Here we show that deep anesthesia with ketamine before euthanasia by decapitation improves the quality of neonatal mouse neuronal brain slice preparations. Specifically we found that neurons of the locus coeruleus (LC) and hypoglossal motor neurons had significantly higher input resistances, and LC neurons that generally are difficult to voltage control, could be more reliably voltage clamped compared to control neurons. |
| Databáze: | OpenAIRE |
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