Fabrication of hydrogel scaffolds via photocrosslinking of methacrylated silk fibroin
| Autor: | V. G. Bogush, M. M. Moisenovich, Maria S Kotliarova, M. N. Kopitsyna, Dmitriy V. Bagrov, A. M. Moysenovich, Evgeny A. Karpushkin, Tatiana N. Bibikova, Аnastasia Yu Arkhipova, Yuri Rochev, Konstantin V. Shaitan, I. V. Bessonov, Igor I. Nikishin, Anna S Soldatenko |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
Formates Cell Survival Photochemistry Polymers Propanols Surface Properties 0206 medical engineering Biomedical Engineering Silk Fibroin Bioengineering Biocompatible Materials 02 engineering and technology Microscopy Atomic Force Biomaterials Mice Tissue engineering Animals Cytoskeleton chemistry.chemical_classification Tissue Engineering Tissue Scaffolds Biomaterial Hydrogels Adhesion Polymer Dynamic mechanical analysis 3T3 Cells 021001 nanoscience & nanotechnology 020601 biomedical engineering Actins Solvent Cross-Linking Reagents Phenotype chemistry Chemical engineering Self-healing hydrogels Methacrylates 0210 nano-technology Fibroins Rheology |
| Zdroj: | Biomedical materials (Bristol, England). 14(3) |
| ISSN: | 1748-605X |
| Popis: | Silk fibroin is a promising biomaterial for tissue engineering due to its valuable mechanical and biological properties. However, being a natural product and a protein, it lacks the processability and uniform quality of an advanced synthetic material. Here we propose a way to overcome this contradiction using novel fibroin photocrosslinkable derivative (FBMA). FBMA was synthesized by methacrylation of native fibroin nucleophilic side groups. It was dissolved in either formic acid (FA) or hexafluoroisopropanol (HFIP), and the obtained solutions were photocrosslinked into hydrogel scaffolds of various structural forms including films, micropatterns, pads and macroporous sponges. UV-exposition of dry FBMA films through a photomask created complex microscaled patterns of the polymer. The nature of the solvent affected the properties of resulting hydrogels. When HFIP was used as the solvent, the resulting hydrogels had a storage modulus ∼4 times higher than that of hydrogels fabricated using FA and ∼20 times higher compared to the reference hydrogel obtained from pristine fibroin. Both FBMA-based hydrogels were biocompatible and supported fibroblast adhesion and growth in vitro. Cells cultivated on FBMA scaffolds produced with HFIP exhibited more spread phenotype at 4 and 24 h of cultivation, consistent with increased stiffness of the hydrogel. Hence, FBMA is an attractive material for fabrication of micropatterned scaffolds of centimeter-scale size with minutely tunable physico-chemical properties via convenient and reproducible technological processes, applicable for rapid prototyping. |
| Databáze: | OpenAIRE |
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