Characterisation and differential expression during development of a duplicate Disabled-1 (Dab1) gene from zebrafish
| Autor: | Rosario Arévalo, M. Javier Herrero-Turrión, José Aijón, Juan M. Lara, Almudena Velasco |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Gene isoform Physiology Molecular Sequence Data Biochemistry Evolution Molecular Mice Exon Genes Duplicate Gene expression Gene duplication Animals Humans Protein Isoforms Amino Acid Sequence Molecular Biology Zebrafish Gene Conserved Sequence Adaptor Proteins Signal Transducing Genetics Base Sequence biology Reverse Transcriptase Polymerase Chain Reaction Alternative splicing Computational Biology Gene Expression Regulation Developmental Genomics Zebrafish Proteins biology.organism_classification Rats genomic DNA Female |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 155:217-229 |
| ISSN: | 1096-4959 |
| DOI: | 10.1016/j.cbpb.2009.11.003 |
| Popis: | We identified a new duplicated Dab1 gene (drDab1b) spanning around 25kb of genomic DNA in zebrafish. Located in zebrafish chromosome 2, it is composed of 11 encoding exons and shows high sequence similarity to other Dab1 genes, including drDab1a, a zebrafish Dab1 gene previously characterised. drDab1b encodes by alternative splicing at least five different isoforms. Both drDab1a and drDab1b show differential gene expression levels in distinct adult tissues and during development. drDab1b is expressed in peripheral tissues (gills, heart, intestine, muscle), the immune system (blood, liver) and the central nervous system (CNS), whereas drDab1a is only expressed in gills, muscle and the CNS, suggesting a division of functions for two Dab1 genes in zebrafish adult tissues. RT-PCR analysis also reveals that both drDab1 genes show distinct developmental-specific expression patterns throughout development. drDab1b expression was higher than that of drDab1a, suggesting a major role of drDab1b in comparison with drDab1a during development and in different adult tissues. In addition, new putative Dab1 (a and/or b) from different teleost species were identified in silico and predicted protein products are compared with the previously characterised Dab1, demonstrating that the Dab1b group is more ancestral than their paralogue, the Dab1a group. |
| Databáze: | OpenAIRE |
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