Molecular mechanisms of colistin resistance among Klebsiella pneumoniae strains
| Autor: | Gökhan Aygün, Barış Ata Borsa, Zeynep Gungordu-Dalar, Goktug Karabiyik, Mehmet Demirci, Ömer Küçükbasmacı |
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| Přispěvatelé: | B.A. Borsa, M. Demirci, Z. Gungordu-Dalar, G. Karabiyik, G. Aygun, O. Kucukbasmaci, Yeditepe Üniversitesi, Güngördü-Dalar, Zeynep |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Klebsiella pneumoniae
medicine.drug_class Antibiotics Resistance Drug resistance Microbial Sensitivity Tests General Biochemistry Genetics and Molecular Biology Bacterial genetics Microbiology Plasmid Bacterial Proteins Drug Resistance Bacterial medicine polycyclic compounds Humans biology Colistin Broth microdilution Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition biology.organism_classification equipment and supplies Anti-Bacterial Agents Klebsiella Infections Mutation bacteria lipids (amino acids peptides and proteins) Bacteria medicine.drug |
| Popis: | Demirci, Mehmet/0000-0001-9670-2426; Karabiyik, Goktug/0000-0002-0396-7626; GUNGORDU DALAR, Zeynep/0000-0003-2177-4235; Kucukbasmaci, Omer/0000-0002-8678-4218; Aygun, Gokhan/0000-0001-6915-9843 WOS:000475700400001 PubMed: 31307167 Background: The increasing rate of infections caused by multiple drug resistant gram-negative bacteria has led to resuscitation of colistin. As a result, colistin resistance, mainly among Klebsiella pneumoniae strains has also been increased. The aim of this study was to investigate molecular mechanisms behind colistin resistance. Methods: Twenty colistin-resistant K. pneumoniae strains isolated from clinical samples of different patients were involved in this study. VITEK2 automated ID/AST system (Biomeriux, France) was used for the identification and also the susceptibility testing for antibiotics other than colistin. Colistin susceptibility was determined by broth microdilution method. To identify the mechanisms of resistance, mutations on mgrB genes, expression levels of pmrA, pmrB, pmrC, pmrD, pmrE, pmrK, phoQ, and phoP genes, and the presence of plasmid mediated colistin resistance genes, mcr-1 and mcr-2 were investigated. Results: As a result of the study, increased expression levels of the pmrA, pmrB, pmrD, pmrK, phoP, and phoQ genes were observed. All colistin resistant strains were found wild type for the mgrB gene which is thought to be responsible for colistin resistance. Also, no mcr-1 or mcr-2 genes which are the causes of plasmid mediated colistin resistance have been detected in any of the strains. Conclusions: Among the colistin resistant K. pneumoniae strains included in our study, increased expression levels of the genes responsible for cell membrane modifications related with colistin resistance were the most common mechanisms. Istanbul University Scientific Research Projects UnitIstanbul University [57713] This study was supported by the Istanbul University Scientific Research Projects Unit, Project No. 57713. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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