Neuronal morphology and synaptic plasticity in the hippocampus of vitamin A deficient rats
| Autor: | Serge Alfos, Clémentine Bosch-Bouju, Véronique Pallet, Fabien Dumetz, Rachel Ginieis, Anaïs Marie, Corinne Buré |
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| Přispěvatelé: | Nutrition et Neurobiologie intégrée (NutriNeuro), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Ecole nationale supérieure de chimie, biologie et physique-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Vitamin Male medicine.medical_specialty [SDV]Life Sciences [q-bio] Retinoic acid Medicine (miscellaneous) Hippocampus Hippocampal formation Biology memory 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Tandem Mass Spectrometry Internal medicine medicine retinoic acid Animals Rats Wistar Vitamin A Tissue homeostasis Neurons 030109 nutrition & dietetics Nutrition and Dietetics Neuronal Plasticity General Neuroscience Retinol General Medicine medicine.disease Rats Vitamin A deficiency Endocrinology chemistry synaptic network Synaptic plasticity neuron morphology 030217 neurology & neurosurgery Chromatography Liquid |
| Zdroj: | Nutritional Neuroscience Nutritional Neuroscience, Maney Publishing, In press, pp.1-12. ⟨10.1080/1028415X.2020.1809877⟩ |
| ISSN: | 1476-8305 1028-415X |
| Popis: | Vitamin A (retinol) and related retinoids are micronutrients provided by food. Retinol derivatives are growth factors important for development, cell differentiation and tissue homeostasis, especially in the brain. Objective: The hippocampus is a pivotal brain structure for learning and memory and hippocampal-dependent memory is highly sensitive to retinoids action. However, the underlying mechanisms are still unclear. In this study, we characterized the impact of vitamin A deficiency on memory and neuronal plasticity, focusing on the CA1 region of the hippocampus in rats. Methods: Weaned male Wistar rats were fed a control (5 UI/g) or deficient vitamin A diet (0 UI/g) for 10 weeks. The effect of vitamin A supplementation (20 UI/g) for 3 weeks was also tested. Memory performances were assessed in the Y-maze (n = 24-30/group), retinoic acid levels were measured (LC-MS/MS) in the serum and in the hippocampus (n = 5/group), CA1 neuronal architecture was analyzed with Golgi staining (n = 17-20 neurons/group) and electrophysiological patch-clamp recordings were performed on hippocampal brain slices (n = 6-11/group). Results: Vitamin A deficiency from weaning significantly lowered hippocampal levels of retinoic acid, reduced dendritic length and branching of CA1 pyramidal neurons and decreased spontaneous glutamatergic synaptic events and synaptic plasticity. When replenishment with moderate dose of dietary vitamin A for 3 weeks was done, most of the synaptic and morphological alterations were absent. Conclusion: This study provides new mechanistic insight to understand the critical role of retinoic acid in hippocampal function. |
| Databáze: | OpenAIRE |
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