Histochemical localization of the PBAN receptor in the pheromone gland of Heliothis peltigera
| Autor: | Manuela Martins-Green, Miriam Altstein, Orna Ben-Aziz, Qi-Jing Li, Kalpana Bhargava |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Physiology Moths Biochemistry Pheromones Cellular and Molecular Neuroscience Endocrinology Internal medicine medicine Animals Receptors Invertebrate Peptide Receptor Cuticle (hair) Dose-Response Relationship Drug biology Histocytochemistry Neuropeptides biology.organism_classification Cell biology Heliothis Microscopy Electron Scanning Pheromone biosynthesis activating neuropeptide Pheromone Ovipositor Immunohistochemistry Heliothis peltigera |
| Zdroj: | Peptides. 24:1335-1347 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/j.peptides.2003.09.011 |
| Popis: | The presence of the pyrokinin (PK)/ Pheromone biosynthesis activating neuropeptide (PBAN) receptor in pheromone gland cells of Heliothis peltigera females was demonstrated, and its spatial distribution in the ovipositor was visualized with two photo-affinity biotinilated ligands: BpaPBAN1-33NH 2 and BpaArg 27 -PBAN28-33NH 2 . Light microscopy histological studies revealed that the gland is contained within the inter-segmental membrane (ISM) between the 8th and 9th abdominal segments. The gland was found to be composed of a single layer of columnar epithelial cells positioned under the inter-segmental cuticle. Similar epithelial cells were also found in the dorsal and ventral regions of the 9th abdominal segment. All regions containing the glandular cells bound both ligands, indicating presence of the PK/PBAN receptor. The patterns obtained with both ligands were similar, hinting at the possibility that either both ligands bind to the same receptor, or, that if there are two distinct receptors, their spatial distribution throughout the gland is very similar. |
| Databáze: | OpenAIRE |
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