In situ analysis of sulfur in the sulfur globules of phototrophic sulfur bacteria by X-ray absorption near edge spectroscopy
| Autor: | Christiane Dahl, Hans G. Trüper, Ralf Steudel, Hartwig Modrow, Oliver Schumann, Josef Hormes, Claudia Engemann, Alexander Prange, Isolde Arzberger |
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| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
Thiosulfate Sulfide biology Glutathione Disulfide Spectrum Analysis Inorganic chemistry Biophysics Sulfur metabolism chemistry.chemical_element Chlorobium biology.organism_classification Biochemistry Sulfur Glutathione XANES Halorhodospira halophila Chlorobi chemistry.chemical_compound chemistry Green sulfur bacteria Molecular Biology Electron Probe Microanalysis |
| Zdroj: | Biochimica et biophysica acta. 1428(2-3) |
| ISSN: | 0006-3002 |
| Popis: | During the oxidation of sulfide and thiosulfate purple and green sulfur bacteria accumulate globules of 'elemental' sulfur. Although essential for a thorough understanding of sulfur metabolism in these organisms, the exact chemical nature of the stored sulfur is still unclear. We applied sulfur K-edge X-ray absorption near edge spectroscopy (XANES) to probe the forms of sulfur in intact cells. Comparing XANES spectra of Allochromatium vinosum, Thiocapsa roseopersicina, Marichromatium purpuratum, Halorhodospira halophila and Chlorobium vibrioforme grown photolithoautotrophically on sulfide with reference probes (fingerprint method), we found sulfur chains with the structure R-S(n)-R. Evidence for the presence of sulfur rings, polythionates and anionic polysulfides in the sulfur globules of these bacteria was not obtained. |
| Databáze: | OpenAIRE |
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