Induction of antibiotic specialized metabolism by co‐culturing in a collection of phyllosphere bacteria

Autor: Gabriele M. König, Tom Coenye, Peter Vandamme, Aurélien Carlier, Alexander Bogdanov, Tessa Acar, Margo Cnockaert, Shan Shan Qi, Max Crüsemann, Sarah Ranty-Roby
Přispěvatelé: Universiteit Gent = Ghent University [Belgium] (UGENT), University of Bonn, University of California [San Diego] (UC San Diego), University of California, Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ghent University under grant BOF17/STA/024, Geconcerteerde Onderzoeksacties (GOA) of Ghent University (grant BOF15/GOA/006), Wellcome Trust grant reference 203141/Z/16/Z, Oxford Genomics Centre at the Wellcome Centre for Human Genetics (funded by Wellcome Trust grantreference 203141/Z/16/Z), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Environmental Microbiology
Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2021, 23 (4), pp.2132-2151. ⟨10.1111/1462-2920.15382⟩
ISSN: 1462-2912
1462-2920
DOI: 10.1111/1462‐2920.15382⟩
Popis: International audience; A diverse set of bacteria live on the above ground parts of plants, composing the phyllosphere, and play important roles for plant health. Phyllosphere microbial communities assemble in a predictable manner and diverge from communities colonizing other plant organs or the soil. However, how these communities differ functionally remains obscure. We assembled a collection of 258 bacterial isolates representative of the most abundant taxa of the phyllosphere of Arabidopsis and a shared soil inoculum. We screened the collection for the production of metabolites that inhibit the growth of gram-positive and gram-negative bacteria either in isolation or in co-culture. We found that isolates capable of constitutive antibiotic production in monoculture were significantly enriched in the soil fraction. In contrast, the proportion of binary cultures resulting in the production of growth inhibitory compounds differed only marginally between the phyllosphere and soil fractions. This shows that the phyllosphere may be a rich resource for potentially novel molecules with antibiotic activity, but that production or activity is dependent upon induction by external signals or cues. Finally, we describe the isolation of antimicrobial acyloin metabolites from a binary culture of Arabidopsis phyllosphere isolates, which inhibit the growth of clinically relevant Acinetobacter baumannii.
Databáze: OpenAIRE
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